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CD300a Rabbit Polyclonal Antibody

CMRF35-like molecule 8,CLM-8,CD300 antigen-like family member A,CMRF-35-H9 (CMRF35-H9),CMRF35-H,IRC1/IRC2,Immunoglobulin superfamily member 12 (IgSF12),CMRF35H,IGSF12

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B1473

规格

25μl100μl1ml

数量

产品介绍

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产品规格

宿主来源

Rabbit
抗原名称

CD300a
分子别名

CMRF35-like molecule 8; CLM-8; CD300 antigen-like family member A; CMRF-35-H9 (CMRF35-H9); CMRF35-H; IRC1/IRC2; Immunoglobulin superfamily member 12 (IgSF12); CMRF35H; IGSF12
免疫原

Recombinant Protein
细胞定位

Cell membrane
Accession

Q9UGN4
抗体类型

Polyclonal antibody
抗体同种型

IgG
反应种属 ?

Hu
阳性样本

THP-1, U-937, HL-60
纯化方式

Immunogen Affinity
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

FCM

WB
稀释度

应用稀释度推荐种属

WB 1:500-1:1000 Hu
FCM 1:500 Hu

应用	稀释度	推荐种属
WB	1:500-1:1000	Hu
FCM	1:500	Hu

背景介绍

CD300a protein is a member of the CD300 glycoprotein family, which are type I transmembrane proteins expressed on the surface of various leukocytes. It has an IgV-like extracellular domain and a long cytoplasmic tail containing immunoreceptor tyrosine-based inhibitory motifs (ITIMs), which are crucial for its inhibitory signaling. CD300a is expressed in natural killer (NK) cells, subsets of T and B cells, monocytes, dendritic cells, granulocytes, and mast cells. It plays an inhibitory role in immune responses by regulating various functions such as inhibiting Ca++ mobilization in response to receptor stimulation, suppressing NK cell-mediated cytotoxicity, B-cell proliferation, and mast cell activation. Additionally, CD300a mediates immune tolerance to dying cells by binding to phosphatidylserine and phosphatidylethanolamine. Its expression and function are regulated by various cytokines and stimuli, and it has potential implications in allergic diseases and other immune-related conditions.

免疫印迹
- WB result of CD300a Rabbit pAb
  Primary antibody: CD300a Rabbit pAb at 1/1000 dilution
  Lane 1: 293T whole cell lysate 20 µg
  Lane 2: THP-1 whole cell lysate 20 µg
  Lane 3: U-937 whole cell lysate 20 µg
  Lane 4: HL-60 whole cell lysate 20 µg
  Negative control: 293T whole cell lysate
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 33 kDa
  Observed MW: 40-100 kDa
流式分析
- Flow cytometric analysis of Human PBMC (human peripheral blood mononuclear cells) labelling CD300a antibody at 1/500 (0.1 μg) dilution/ (Right panel) compared with a Rabbit IgG Isotype Control / (left panel). Goat Anti-Rabbit DyLight® 488 was used as the secondary antibody. Then cells were stained with CD14 - Brilliant Violet 421™ antibody separately.