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Rabbit抗原名称
CA2分子别名
Carbonic anhydrase 2; Carbonate dehydratase II; Carbonic anhydrase C (CAC); Carbonic anhydrase II (CA-II); Cyanamide hydratase CA2免疫原
Synthetic Peptide细胞定位
Cytoplasm, Cell membraneAccession
P00918抗体类型
Polyclonal antibody抗体同种型
IgG反应种属 ?
Hu, Ms, Rt阳性样本
Caco-2, MCF7, A431, HEK-293, mouse brain, rat brain纯化方式
Immunogen Affinity浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu, Ms, Rt IHC-P 1:1000 Hu, Ms, Rt ICC 1:500 Hu
Carbonic anhydrase 2 (CA2) is a zinc-containing metalloenzyme that belongs to the carbonic anhydrase family and plays a crucial role in catalyzing the reversible hydration of carbon dioxide (CO₂) to bicarbonate (HCO₃⁻) and protons (H⁺). This reaction is essential for maintaining pH homeostasis, electrolyte balance, and CO₂ transport in various tissues, including the kidneys, red blood cells, and the central nervous system. CA2 is one of the most efficient enzymes, with a catalytic rate approaching diffusion limits, and its activity is critical for physiological processes such as respiration, bone resorption, renal acid secretion, and cerebrospinal fluid production. Mutations in the CA2 gene can lead to osteopetrosis with renal tubular acidosis and cerebral calcification, highlighting its importance in bone remodeling and acid-base regulation. Additionally, CA2 is implicated in pathological conditions, including cancer, glaucoma, and epilepsy, making it a potential therapeutic target for inhibitors like acetazolamide. Its structure consists of a conserved α-carbonic anhydrase fold with a zinc ion coordinated by three histidine residues in the active site, enabling rapid proton transfer via a water-mediated mechanism.
免疫印迹
WB result of CA2 Rabbit pAb
Primary antibody: CA2 Rabbit pAb at 1/1000 dilution
Lane 1: Caco-2 whole cell lysate 20 µg
Lane 2: MCF7 whole cell lysate 20 µg
Lane 3: A431 whole cell lysate 20 µg
Lane 4: HEK-293 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 29 kDa
Observed MW: 35 kDaWB result of CA2 Rabbit pAb
Primary antibody: CA2 Rabbit pAb at 1/1000 dilution
Lane 1: mouse brain lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 29 kDa
Observed MW: 35 kDaWB result of CA2 Rabbit pAb
Primary antibody: CA2 Rabbit pAb at 1/1000 dilution
Lane 1: rat brain lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 29 kDa
Observed MW: 35 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human colon. Anti-CA2 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human stomach. Anti-CA2 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human gastrointestinal stromal tumor. Anti-CA2 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-CA2 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human renal clear cell carcinoma. Anti-CA2 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-CA2 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti-CA2 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat kidney. Anti-CA2 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
ICC shows positive staining in MCF7 cells. Anti-CA2 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
组织表达图谱
Expression of CA2 in tumor tissue.
Expression of CA2 in human tissue.
Expression of CA2 in mouse & rat tissue.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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