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Rabbit抗原名称
CD112R分子别名
Transmembrane protein PVRIG; CD112 receptor (CD112R); Poliovirus receptor-related immunoglobulin domain-containing protein; C7orf15; PVRIG免疫原
Recombinant Protein细胞定位
Cell membraneAccession
Q6DKI7克隆号
S-1923-22抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu阳性样本
Jurkat, Daudi, Ramos纯化方式
Protein A浓度
1 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu
CD112R, also known as PVRIG, is a co-inhibitory receptor belonging to the poliovirus receptor (PVR) family. It is a 36kD single-pass transmembrane protein composed of one transmembrane domain, a long intracellular domain, and a single extracellular immunoglobulin variable-like (IgV) domain. CD112R is predominantly expressed on natural killer (NK) cells and T cells, especially CD8+ T cells, and its expression is upregulated upon activation. In the tumor microenvironment, CD112R is highly expressed on NK cells, CD4+ T cells, and CD8+ T cells. It binds to its ligand CD112 (NECTIN2 or PVRL2) with high affinity, inhibiting the activation of T cells and NK cells, thus contributing to immune evasion in cancer. This makes the CD112R/CD112 axis a potential target for cancer immunotherapy.
免疫印迹
WB result of CD112R Recombinant Rabbit mAb
Primary antibody: CD112R Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: Molt-4 whole cell lysate 20 µg
Lane 3: Jurkat whole cell lysate 20 µg
Lane 4: Daudi whole cell lysate 20 µg
Lane 5: Ramos whole cell lysate 20 µg
Negative control: HeLa whole cell lysate
Weak expression: Molt-4 whole cell lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 34 kDa
Observed MW: 35 kDa
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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