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Rabbit抗原名称
AQR/IBP160分子别名
RNA helicase aquarius; Intron-binding protein of 160 kDa (IBP160); KIAA0560免疫原
Synthetic Peptide细胞定位
NucleusAccession
O60306克隆号
S-1329-248抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu阳性样本
HeLa, HEK-293, HepG2纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
ICC ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu ICC 1:500 Hu
AQR/IBP160 protein, also known as Aquarius homolog or Intron-binding protein of 160 kDa, is a spliceosomal intron-binding protein involved in pre-mRNA splicing and small nucleolar ribonucleoprotein (snoRNP) biogenesis. It links the position-dependent assembly of intron-encoded box C/D snoRNPs to pre-mRNA splicing, playing a key role in the folding of snoRNA sequences. This protein has ATP-dependent RNA helicase activity and can unwind double-stranded RNA molecules with a 3' overhang. It binds to introns of pre-mRNAs in a sequence-independent manner, contacting the region between snoRNA and the branchpoint of introns during the late stages of splicing.
免疫印迹
WB result of AQR/IBP160 Recombinant Rabbit mAb
Primary antibody: AQR/IBP160 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: HEK-293 whole cell lysate 20 µg
Lane 3: HepG2 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 171 kDa
Observed MW: 180 kDa
免疫细胞化学
ICC shows positive staining in HepG2 cells. Anti- AQR/IBP160 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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