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Phospho-TGF β Receptor I(Ser165) Recombinant Rabbit mAb (S-1896-21)

TGF-beta receptor type-1,TGFR-1,Activin A receptor type II-like protein kinase of 53kD,Activin receptor-like kinase 5 (ALK-5,ALK5),Serine/threonine-protein kinase receptor R4 (SKR4),TGF-beta type I receptor,Transforming growth factor-beta receptor type I (TGF-beta receptor type I,TbetaR-I),ALK5,SKR4,TGFBR1

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B1463

规格

25μl100μl1ml

数量

产品介绍

产品介绍 FAQs 评论(0)

产品规格

宿主来源

Rabbit
抗原名称

Phospho-TGF β Receptor I(Ser165)
分子别名

TGF-beta receptor type-1; TGFR-1; Activin A receptor type II-like protein kinase of 53kD; Activin receptor-like kinase 5 (ALK-5; ALK5); Serine/threonine-protein kinase receptor R4 (SKR4); TGF-beta type I receptor; Transforming growth factor-beta receptor type I (TGF-beta receptor type I; TbetaR-I); ALK5; SKR4; TGFBR1
免疫原

Synthetic Peptide
细胞定位

Cell membrane
Accession

P36897
克隆号

S-1896-21
抗体类型

Recombinant mAb
抗体同种型

IgG
翻译后修饰类型

磷酸化
反应种属 ?

Hu, Ms
阳性样本

HeLa treated with 100 ng/ml Calyculin A for 30 minutes, Serum-starved NIH/3T3 treated with 100 nM Calyculin A for 30 minutes
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

ICC ?

WB
稀释度

应用稀释度推荐种属

Dot Blot 1:1000
WB 1:1000 Hu, Ms
ICC 1:500 Hu

应用	稀释度	推荐种属
Dot Blot	1:1000
WB	1:1000	Hu, Ms
ICC	1:500	Hu

背景介绍

Phospho-TGF β Receptor I (Ser165) is a phosphorylated form of the Transforming Growth Factor-beta Receptor Type I (TGFBR1), a transmembrane serine/threonine kinase that plays a crucial role in transmitting TGF-beta signals from the cell surface to the cytoplasm. This receptor forms a complex with TGF-beta type II receptors and is activated upon binding to TGF-beta ligands, leading to the phosphorylation of downstream SMAD proteins, which then regulate gene transcription involved in cell cycle control, differentiation, wound healing, and immune responses. The phosphorylation at Ser165 is particularly important for its activation and signaling, and this site is often targeted by antibodies for research applications such as Western blotting, immunohistochemistry, and immunofluorescence.

免疫印迹
- WB result of Phospho-TGF β Receptor I(Ser165) Recombinant Rabbit mAb
  Primary antibody: Phospho-TGF β Receptor I(Ser165) Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: untreated HeLa whole cell lysate 20 µg
  Lane 2: HeLa treated with 100 ng/ml Calyculin A for 30 minutes whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 56 kDa
  Observed MW: 60 kDa
- WB result of Phospho-TGF β Receptor I(Ser165) Recombinant Rabbit mAb
  Primary antibody: Phospho-TGF β Receptor I(Ser165) Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: untreated NIH/3T3 whole cell lysate 20 µg
  Lane 2: serum-starved NIH/3T3 treated with 100 nM Calyculin A for 30 minutes whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 56 kDa
  Observed MW: 60 kDa
斑点杂交
- Dot blot result of Phospho-TGF β Receptor I(Ser165) Recombinant Rabbit mAb
  Lane 1: TGF β Receptor I (Ser165) phospho peptide
  Lane 2: TGF β Receptor I (Ser165) unmodified peptide
  Primary antibody: Phospho-TGF β Receptor I(Ser165) Recombinant Rabbit mAb at 1/1000 dilution
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
免疫细胞化学
- ICC analysis of HeLa cells treated with 100 ng/ml Calyculin A for 30 minutes (top panel) and untreated HeLa cells (below panel). Anti- Phospho-TGF β Receptor I(Ser165) antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).