PDIA3 Rabbit Polyclonal Antibody
Protein disulfide-isomerase A3,58 kDa glucose-regulated protein,58 kDa microsomal protein (p58),Disulfide isomerase ER-60,Endoplasmic reticulum resident protein 57 (ER protein 57,ERp57),Endoplasmic reticulum resident protein 60 (ER protein 60,ERp60),ERP57,ERP60,GRP58
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宿主来源
Rabbit抗原名称
PDIA3分子别名
Protein disulfide-isomerase A3; 58 kDa glucose-regulated protein; 58 kDa microsomal protein (p58); Disulfide isomerase ER-60; Endoplasmic reticulum resident protein 57 (ER protein 57; ERp57); Endoplasmic reticulum resident protein 60 (ER protein 60; ERp60); ERP57; ERP60; GRP58免疫原
Synthetic Peptide细胞定位
Endoplasmic reticulumAccession
P30101抗体类型
Polyclonal antibody抗体同种型
IgG反应种属 ?
Hu阳性样本
HepG2, Daudi, HeLa, HaCat, 293T, PC-3, human colon, human colon cancer, human endometrium cancer, human lung cancer, human thyroid cancer预测反应种属
(反应种属缩写表)Bv, AfGrMk纯化方式
Immunogen Affinity浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
ICFCM
IHC-P ?
ICC ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu IHC-P 1:250 Hu ICC 1:500 Hu ICFCM 1:500 Hu
PDIA3 (Protein Disulfide Isomerase Family A Member 3), also known as ERp57 or GRP58, is a multifunctional enzyme belonging to the protein disulfide isomerase (PDI) family. It is predominantly located in the endoplasmic reticulum (ER) and acts as a molecular chaperone and redox catalyst. PDIA3 interacts with lectin chaperones like calreticulin and calnexin to facilitate the folding of newly synthesized glycoproteins by promoting the formation of disulfide bonds. Additionally, it prevents protein aggregation and plays a role in the unfolded protein response (UPR) during ER stress. Beyond its canonical functions, PDIA3 has been implicated in various biological processes, including viral infections, where it can influence viral entry and replication. In disease contexts, PDIA3 is often upregulated in cancers and has been associated with tumor progression and drug resistance. Recent studies also highlight its involvement in metabolic disorders, such as obesity, by regulating the function of adipose tissue macrophages.
免疫印迹
WB result of PDIA3 Rabbit pAb
Primary antibody: PDIA3 Rabbit pAb at 1/1000 dilution
Lane 1: HepG2 whole cell lysate 20 µg
Lane 2: Daudi whole cell lysate 20 µg
Lane 3: HeLa whole cell lysate 20 µg
Lane 4: HaCaT whole cell lysate 20 µg
Lane 5: 293T whole cell lysate 20 µg
Lane 6: PC-3 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 57 kDa
Observed MW: 62 kDa
流式分析
Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized Daudi (Human Burkitt's lymphoma lymphoblast) labelling PDIA3 antibody at 1/500 dilution (0.1 μg) / (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
免疫组化
IHC shows positive staining in paraffin-embedded human colon. Anti-PDIA3 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-PDIA3 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human endometrium cancer. Anti-PDIA3 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung cancer. Anti-PDIA3 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human thyroid cancer. Anti-PDIA3 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
ICC shows positive staining in Daudi cells. Anti- PDIA3 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
组织表达图谱
Expression of PDIA3 in tumor tissue.
Expression of PDIA3 in human tissue.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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