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宿主来源
Rabbit抗原名称
TRP2/DCT分子别名
L-dopachrome tautomerase; DCT; DT; L-dopachrome Delta-isomerase; Tyrosinase-related protein 2 (TRP-2; TRP2); TYRP2免疫原
Synthetic Peptide细胞定位
MelanosomeAccession
P40126克隆号
S-1749-19抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu阳性样本
SK-MEL-2, SK-MEL-28纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu
Tyrosinase-related protein-2 (TRP2), also known as Dopachrome tautomerase (DCT), is a key enzyme in the melanin biosynthetic pathway, primarily expressed in melanocytes, the pigment-producing cells. It is a member of the tyrosinase-related protein family and plays a crucial role in melanogenesis by converting Dopachrome to 5,6-dihydroxyindole-2-carboxylic acid (DHICA), which is further oxidized to form melanin. This enzyme not only influences the quality and solubility of melanin but also contributes to cellular detoxification and stress resistance by modulating the production of cytotoxic and antioxidant melanin precursors. Additionally, DCT has been implicated in various biological processes, including protection against UV radiation and reactive oxygen species (ROS) damage in melanocytes. Mutations in the DCT gene can lead to oculocutaneous albinism type VIII (OCA8), highlighting its importance in pigmentation.
免疫印迹
WB result of TRP2/DCT Recombinant Rabbit mAb
Primary antibody: TRP2/DCT Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: SK-MEL-2 whole cell lysate 20 µg
Lane 2: SK-MEL-28 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 59 kDa
Observed MW: 65 kDa
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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