PML Recombinant Rabbit mAb (S-1908-60)
Protein PML,E3 SUMO-protein ligase PML,Promyelocytic leukemia protein,RING finger protein 71,RING-type E3 SUMO transferase PML,Tripartite motif-containing protein 19 (TRIM19),MYL,PP8675,RNF71,TRIM19
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宿主来源
Rabbit抗原名称
PML分子别名
Protein PML; E3 SUMO-protein ligase PML; Promyelocytic leukemia protein; RING finger protein 71; RING-type E3 SUMO transferase PML; Tripartite motif-containing protein 19 (TRIM19); MYL; PP8675; RNF71; TRIM19免疫原
Recombinant Protein细胞定位
Nucleus, Endosome, Endoplasmic reticulum, CytoplasmAccession
P29590克隆号
S-1908-60抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu阳性样本
HUVEC, 293T, HeLa, K562, human breast cancer, human lung squamous carcinoma, human prostate cancer, human testis预测反应种属
(反应种属缩写表)/纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
ICFCM
IHC-P ?
ICC ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu IHC-P 1:200 Hu ICC 1:200 Hu ICFCM 1:500 Hu
Promyelocytic leukemia protein (PML), also known as TRIM19, is a multifunctional protein belonging to the TRIM family and is best known for its role in the formation and stability of PML nuclear bodies (PML-NBs) within the cell nucleus. These nuclear bodies are membraneless organelles that serve as hubs for various cellular processes, including transcriptional regulation, DNA damage repair, apoptosis, and antiviral defense. PML itself is a 70 kDa protein with an N-terminal RBCC motif (RING, B-box, and coiled-coil domains) that facilitates its oligomerization and interaction with over 160 other proteins. The protein is also subject to post-translational modifications such as SUMOylation, phosphorylation, and ubiquitination, which regulate its function and localization. PML has been implicated as a tumor suppressor, with its downregulation observed in various cancers, highlighting its importance in maintaining cellular homeostasis and preventing carcinogenesis.
免疫印迹
WB result of PML Recombinant Rabbit mAb
Primary antibody: PML Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HUVEC whole cell lysate 20 µg
Lane 2: 293T whole cell lysate 20 µg
Lane 3: HeLa whole cell lysate 20 µg
Lane 4: K562 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 50-110 kDa
Observed MW: 45-130 kDa
流式分析
Flow cytometric analysis of K562 (Human chronic myelogenous leukemia lymphoblast) labelling PML antibody at 1/500 dilution (0.1 μg) / (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
免疫组化
IHC shows positive staining in paraffin-embedded human breast cancer. Anti-PML antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung squamous carcinoma. Anti-PML antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human prostate cancer. Anti-PML antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human testis. Anti-PML antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
ICC shows positive staining in Jurkat cells. Anti- PML antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
组织表达图谱
Expression of PML in tumor tissue.
Expression of PML in human tissue.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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