Phospho-EGFR (Tyr1173) Recombinant Rabbit mAb (S-1187-106)

Epidermal growth factor receptor,Proto-oncogene c-ErbB-1,Receptor tyrosine-protein kinase erbB-1,ERBB,ERBB1,HER1,EGFR

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B1455

规格

25μl100μl1ml

数量

产品介绍

产品介绍 FAQs 评论(0)

产品规格

宿主来源

Rabbit
抗原名称

Phospho-EGFR (Tyr1173)
分子别名

Epidermal growth factor receptor; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1; ERBB; ERBB1; HER1; EGFR
免疫原

Synthetic Peptide
细胞定位

Cytoplasm, Cell membrane
Accession

P00533
克隆号

S-1187-106
抗体类型

Recombinant mAb
抗体同种型

IgG
翻译后修饰类型

磷酸化
反应种属 ?

Hu
阳性样本

A431 treated with 200 ng/ml EGF for 10 minutes
预测反应种属
(反应种属缩写表)

Mq
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

ICC ?

WB
稀释度

应用稀释度推荐种属

Dot Blot 1:1000
WB 1:1000 Hu
ICC 1:500 Hu

背景介绍

Phospho-EGFR (Tyr1173) is a phosphorylated form of the epidermal growth factor receptor (EGFR), a 170 kDa transmembrane receptor tyrosine kinase that plays a crucial role in cell signaling. When EGFR binds to its ligands, such as EGF, it undergoes dimerization and autophosphorylation, activating multiple downstream signaling pathways. Phosphorylation at Tyr1173 is particularly important as it provides a docking site for proteins like the tyrosine phosphatase SHP-1, which can modulate EGFR activity and downstream signaling. This site is also involved in the MAP kinase signaling pathway, which regulates cell growth, survival, and transformation. Abnormal regulation of EGFR, including phosphorylation at Tyr1173, is often associated with various cancers, making it a key target for therapeutic interventions.

免疫印迹
- WB result of Phospho-EGFR (Tyr1173) Recombinant Rabbit mAb
  Primary antibody: Phospho-EGFR (Tyr1173) Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: untreated A431 whole cell lysate 20 µg
  Lane 2: A431 treated with 200 ng/ml EGF for 10 minutes whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 134 kDa
  Observed MW: 180 kDa
  This blot was developed with high sensitivity substrate
斑点杂交
- Dot blot result of Phospho-EGFR (Tyr1173) Recombinant Rabbit mAb
  Lane 1: EGFR (Tyr1173) phospho peptide
  Lane 2: EGFR (Tyr1173) unmodified peptide
  Primary antibody: Phospho-EGFR (Tyr1173) Recombinant Rabbit mAb at 1/1000 dilution
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
免疫细胞化学
- ICC analysis of A431 cells treated with EGF (200 ng/mL for 10 min) (top panel) and A431 cells untreated with EGF (200 ng/mL for 10 min) (below panel). Anti- Phospho-EGFR (Tyr1173) antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).