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Rabbit抗原名称
eEF2分子别名
Elongation factor 2; EF-2; EF2; EEF2免疫原
Synthetic Peptide细胞定位
Cytoplasm, NucleusAccession
P13639克隆号
S-1976-35抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt阳性样本
HeLa, Jurkat, A431, HEK-293, NIH/3T3, C6, human breast cancer, human cervix cancer, human stomach cancer, human colon, human liver预测反应种属
(反应种属缩写表)Zf, Ck, Bv, Mrst, Hm纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
ICFCM
IHC-P ?
ICC ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu, Ms, Rt IHC-P 1:200 Hu, Ms, Rt ICC 1:500 Hu ICFCM 1:500 Hu
Eukaryotic elongation factor 2 (eEF2) is a crucial regulator of protein synthesis, belonging to the highly conserved guanosine triphosphatase (GTPase) family. It is essential for the elongation phase of protein translation, facilitating the GTP-dependent translocation of the nascent protein chain from the A-site to the P-site of the ribosome. eEF2's activity is tightly regulated by phosphorylation at Thr56 by eEF2 kinase (eEF2K), which inhibits its function and thus suppresses protein synthesis. This protein is ubiquitously expressed in various tissues and plays a significant role in normal cellular functions. However, its dysregulation is implicated in several diseases, including cancer, where overexpression of eEF2 is associated with increased tumor incidence and poorer patient prognosis.
免疫印迹
WB result of eEF2 Recombinant Rabbit mAb
Primary antibody: eEF2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: Jurkat whole cell lysate 20 µg
Lane 3: A431 whole cell lysate 20 µg
Lane 4: HEK-293 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 95 kDa
Observed MW: 110 kDaWB result of eEF2 Recombinant Rabbit mAb
Primary antibody: eEF2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 95 kDa
Observed MW: 110 kDaWB result of eEF2 Recombinant Rabbit mAb
Primary antibody: eEF2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: C6 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 95 kDa
Observed MW: 110 kDa
流式分析
Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) labelling eEF2 antibody at 1/500 dilution (0.1 μg)/ (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
免疫组化
IHC shows positive staining in paraffin-embedded human breast cancer. Anti-eEF2 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cervix cancer. Anti-eEF2 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human stomach cancer. Anti-eEF2 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon. Anti-eEF2 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human liver. Anti-eEF2 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
ICC shows positive staining in HEK-293 cells. Anti- eEF2 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
组织表达图谱
Expression of eEF2 in tumor tissue.
Expression of eEF2 in human tissue.
Expression of eEF2 in mouse and rat tissue.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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