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Rabbit抗原名称
Clathrin heavy chain分子别名
Clathrin heavy chain 1; Clathrin heavy chain on chromosome 17 (CLH-17); CLH17; CLTCL2; KIAA0034; CLTC免疫原
Synthetic Peptide细胞定位
Cytoplasm, CytoskeletonAccession
Q00610克隆号
S-1690-25抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt阳性样本
HeLa, A431, SH-SY5Y, 293T, NIH/3T3, Neuro-2a, PC-12, C6纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu, Ms, Rt IHC-P 1:1000 Hu, Ms, Rt
The clathrin heavy chain (Chc) is a crucial component of the clathrin protein complex, which plays a central role in clathrin-mediated endocytosis, a fundamental cellular process for internalizing various cargoes such as membrane proteins, nutrients, and even pathogens. With a molecular weight of approximately 190 kDa, the clathrin heavy chain forms a triskelion structure along with three clathrin light chains, providing the structural backbone of the clathrin lattice. This lattice can form cages of different sizes and shapes, essential for the invagination and excision of vesicles during endocytosis. The clathrin heavy chain contains 145-residue repeats known as Clathrin Heavy Chain Repeats, which fold into elongated superhelical structures and define distinct domains, including the proximal, knee, distal, and ankle domains. Its N-terminal domain (NTD) adopts a WD40 beta-propeller structure and is vital for interacting with various adaptor proteins (APs) that link the clathrin scaffold to the membrane. These interactions are mediated through multiple binding sites, or "boxes," on the NTD, including the Clathrin box, W-box, and Arrestin box, each recognizing specific motifs on the adaptor proteins.
免疫印迹
WB result of Clathrin heavy chain Recombinant Rabbit mAb
Primary antibody: Clathrin heavy chain Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: A431 whole cell lysate 20 µg
Lane 3: SH-SY5Y whole cell lysate 20 µg
Lane 4: 293T whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 192 kDa
Observed MW: 192 kDaWB result of Clathrin heavy chain Recombinant Rabbit mAb
Primary antibody: Clathrin heavy chain Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: Neuro-2a whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 192 kDa
Observed MW: 192 kDaWB result of Clathrin heavy chain Recombinant Rabbit mAb
Primary antibody: Clathrin heavy chain Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: PC-12 whole cell lysate 20 µg
Lane 2: C6 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 192 kDa
Observed MW: 192 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human colon. Anti-Clathrin heavy chain antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human kidney. Anti-Clathrin heavy chain antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human testis. Anti-Clathrin heavy chain antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast cancer. Anti-Clathrin heavy chain antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-Clathrin heavy chain antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse colon. Anti-Clathrin heavy chain antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti-Clathrin heavy chain antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat kidney. Anti-Clathrin heavy chain antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of Clathrin heavy chain in tumor tissue.
Expression of Clathrin heavy chain in human tissue.
Expression of Clathrin heavy chain in mouse & rat tissue.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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