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PGC1 beta Recombinant Rabbit mAb (S-1212-146)

Peroxisome proliferator-activated receptor gamma coactivator 1-beta,PGC-1-beta,PPAR-gamma coactivator 1-beta,PPARGC-1-beta,PGC-1-related estrogen receptor alpha coactivator,PERC,PGC1,PGC1B,PPARGC1,PPARGC1B

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B1444

规格

25μl100μl1ml

数量

产品介绍

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产品规格

宿主来源

Rabbit
抗原名称

PGC1 beta
分子别名

Peroxisome proliferator-activated receptor gamma coactivator 1-beta; PGC-1-beta; PPAR-gamma coactivator 1-beta; PPARGC-1-beta; PGC-1-related estrogen receptor alpha coactivator; PERC; PGC1; PGC1B; PPARGC1; PPARGC1B
免疫原

Synthetic Peptide
细胞定位

Nucleus
Accession

Q86YN6
克隆号

S-1212-146
抗体类型

Recombinant mAb
抗体同种型

IgG
反应种属 ?

Hu
阳性样本

MCF7, HeLa, SH-SY5Y
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

IHC-P ?

ICC ?

WB
稀释度

应用稀释度推荐种属

WB 1:1000 Hu
IHC-P 1:1000 Hu
ICC 1:500 Hu

应用	稀释度	推荐种属
WB	1:1000	Hu
IHC-P	1:1000	Hu
ICC	1:500	Hu

背景介绍

PGC-1β (Peroxisome Proliferator-Activated Receptor Gamma Coactivator-1 beta) is a transcriptional coactivator that plays a crucial role in regulating cellular energy metabolism, mitochondrial biogenesis, and lipid homeostasis. It shares significant functional overlap with its more extensively studied counterpart, PGC-1α, but has distinct roles in specific tissues. PGC-1β is involved in enhancing oxidative phosphorylation (OXPHOS) and fatty acid oxidation (FAO) in mitochondria, thereby supporting cellular energy production. In diseases such as cancer, PGC-1β can have dual effects; for instance, in colorectal cancer, it acts as a downstream effector of oncogenic pathways, promoting cell survival and tumor growth. In hepatocellular carcinoma, PGC-1β can both support tumor anabolism by regulating lipid synthesis and contribute to drug resistance through mitochondrial adaptations. Its complex interactions with various transcription factors and metabolic pathways highlight its importance in both physiological and pathological contexts.

免疫印迹
- WB result of PGC1 beta Recombinant Rabbit mAb
  Primary antibody: PGC1 beta Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: MCF7 whole cell lysate 20 µg
  Lane 2: HeLa whole cell lysate 20 µg
  Lane 3: SH-SY5Y whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 113 kDa
  Observed MW: 115 kDa
免疫组化
- IHC shows positive staining in paraffin-embedded human colon. Anti-PGC1 beta antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human prostatic hyperplasia. Anti-PGC1 beta antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human cervical squamous cell carcinoma. Anti-PGC1 beta antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human colon cancer. Anti-PGC1 beta antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
- ICC shows positive staining in MCF7 cells. Anti-PGC1 beta antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
组织表达图谱
- Expression of PGC1 beta in tumor tissue.
- Expression of PGC1 beta in human tissue.