Phospho-p53 (Ser15) Rabbit Polyclonal Antibody

Cellular tumor antigen p53,Antigen NY-CO-13,Phosphoprotein p53,Tumor suppressor p53,P53,TP53

价格￥400.00 （供应商现货： 3-5个工作日）

货号 S0B1438

规格

25μl100μl1ml

数量

产品介绍

产品介绍 FAQs 评论(0)

产品规格

宿主来源

Rabbit
抗原名称

Phospho-p53 (Ser15)
分子别名

Cellular tumor antigen p53; Antigen NY-CO-13; Phosphoprotein p53; Tumor suppressor p53; P53; TP53
免疫原

Synthetic Peptide
细胞定位

Nucleus
Accession

P04637
抗体类型

Polyclonal antibody
抗体同种型

IgG
翻译后修饰类型

磷酸化
反应种属 ?

Hu
纯化方式

Immunogen Affinity
浓度

1 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

ICC ?

WB
稀释度

应用稀释度推荐种属

Dot Blot 1:1000
WB 1:1000-1:2000 Hu
ICC 1:500 Hu

背景介绍

Phospho-p53 (Ser15) is a critical biomarker of p53 activation, playing a key role in cellular responses to DNA damage and stress. When cells experience DNA damage, p53 is phosphorylated at serine 15, which stabilizes the protein and enhances its transcriptional activity. This phosphorylation event triggers a cascade of downstream effects, including cell cycle arrest, DNA repair, and apoptosis, depending on the severity of the damage. In senescent cells, phospho-p53 (Ser15) is often localized in the nucleus, where it interacts with other proteins such as FOXO4 to maintain the senescent state and prevent apoptosis. This regulatory mechanism is crucial for understanding the balance between cellular senescence and apoptosis, and it has significant implications for aging, cancer, and other age-related diseases.

免疫印迹
- WB result of Phospho-p53 (Ser15) Rabbit pAb
  Primary antibody: Phospho-p53 (Ser15) Rabbit pAb at 1/2000 dilution
  Lane 1: untreated A431 whole cell lysate 20 µg
  Lane 2: A431 treated with 1 µg/ml doxorubicin for 24 hours whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 44 kDa
  Observed MW: 50 kDa
斑点杂交
- Dot blot result of Phospho-p53 (Ser15) Rabbit pAb
  Lane 1: p53 (Ser15) phospho peptide
  Lane 2: p53 unmodified peptide
  Primary antibody: Phospho-p53 (Ser15) Rabbit pAb at 1/1000 dilution
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
免疫细胞化学
- ICC analysis of A431 cells treated with doxorubicin (1 µg/ml, 24h) (top panel) and untreated A431 cells (below panel). Anti- Phospho-p53 (Ser15) antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).