分享产品介绍 FAQs 评论(0)
宿主来源
Rabbit抗原名称
Ezrin分子别名
Cytovillin; Villin-2; p81; VIL2; EZR免疫原
Recombinant Protein细胞定位
Cytoplasm, Cytoskeleton, Cell membraneAccession
P15311克隆号
S-1894-4抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt阳性样本
HeLa, Raji, NIH/3T3, C6预测反应种属
(反应种属缩写表)Rb, Bv纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu, Ms, Rt IHC-P 1:1000 Hu, Ms, Rt
Ezrin is a key member of the Ezrin-Radixin-Moesin (ERM) protein family, functioning as a linker protein that connects the plasma membrane to the actin cytoskeleton. It plays a crucial role in mediating membrane-cytoskeleton interactions, regulating cell morphology, adhesion, migration, and survival. Structurally, Ezrin consists of three main domains: an N-terminal FERM domain that binds to membrane proteins and lipids, a central helical linker region, and a C-terminal ERM-associated domain (C-ERMAD) that interacts with actin filaments. In its inactive state, Ezrin is held in the cytoplasm through intramolecular interactions, but it can be activated by phosphorylation at Thr567, leading to its translocation to the plasma membrane. Ezrin is essential for various cellular functions, including the formation of membrane protrusions like microvilli and invadopodia, which are critical for cell motility and invasion. Abnormal regulation of Ezrin has been implicated in several diseases, particularly cancer, where its overexpression or dysregulation can promote tumor progression, invasion, and metastasis.
免疫印迹
WB result of Ezrin Recombinant Rabbit mAb
Primary antibody: Ezrin Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: Raji whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 69 kDa
Observed MW: 80 kDaWB result of Ezrin Recombinant Rabbit mAb
Primary antibody: Ezrin Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 69 kDa
Observed MW: 80 kDaWB result of Ezrin Recombinant Rabbit mAb
Primary antibody: Ezrin Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: C6 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 69 kDa
Observed MW: 80 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human colon. Anti-Ezrin antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast cancer. Anti-Ezrin antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-Ezrin antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse colon. Anti-Ezrin antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat colon. Anti-Ezrin antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of Ezrin in tumor tissue.
Expression of Ezrin in human tissue.
Expression of Ezrin in mouse & rat tissue.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
评论(0)