PI3 Kinase p85 beta Recombinant Rabbit mAb (S-1058-266)
Phosphatidylinositol 3-kinase regulatory subunit beta,PI3-kinase regulatory subunit beta,PI3K regulatory subunit beta,PtdIns-3-kinase regulatory subunit beta,Phosphatidylinositol 3-kinase 85 kDa regulatory subunit beta (PI3-kinase subunit p85-beta,PtdIns-3-kinase regulatory subunit p85-beta),PIK3R2
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宿主来源
Rabbit抗原名称
PI3 Kinase p85 beta分子别名
Phosphatidylinositol 3-kinase regulatory subunit beta; PI3-kinase regulatory subunit beta; PI3K regulatory subunit beta; PtdIns-3-kinase regulatory subunit beta; Phosphatidylinositol 3-kinase 85 kDa regulatory subunit beta (PI3-kinase subunit p85-beta; PtdIns-3-kinase regulatory subunit p85-beta); PIK3R2免疫原
Synthetic Peptide细胞定位
Cytoplasm, NucleusAccession
O00459克隆号
S-1058-266抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu阳性样本
HeLa, Jurkat, HEK-293预测反应种属
(反应种属缩写表)Bv纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
ICC ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu IHC-P 1:250 Hu ICC 1:500 Hu
PI3 Kinase p85 beta is a regulatory subunit of the phosphoinositide 3-kinase (PI3K) complex, which is crucial for generating 3-phosphorylated phosphoinositides that initiate a wide range of signaling pathways. Unlike its counterpart p85 alpha, p85 beta has both overlapping and unique functions. While p85 beta alone does not significantly impact B cell development, it can partially compensate for the loss of p85 alpha in B cell development and peripheral survival. Additionally, p85 beta has been shown to have distinct effects on apoptosis and may play a role in oncogenic transformation in certain cell types. Its presence is essential for maintaining proper signaling balance, as its deletion can lead to increased BCR-mediated proliferation and altered ERK phosphorylation.
免疫印迹
WB result of PI3 Kinase p85 beta Recombinant Rabbit mAb
Primary antibody: PI3 Kinase p85 beta Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: Jurkat whole cell lysate 20 µg
Lane 3: HEK-293 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 82 kDa
Observed MW: 85 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-PI3 Kinase p85 beta antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human testis. Anti-PI3 Kinase p85 beta antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast cancer. Anti-PI3 Kinase p85 beta antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cervical squamous cell carcinoma. Anti-PI3 Kinase p85 beta antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
ICC shows positive staining in HeLa cells. Anti- PI3 Kinase p85 beta antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
组织表达图谱
Expression of PI3 Kinase p85 beta in human tissue.
Expression of PI3 Kinase p85 beta in tumor tissue.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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