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Rabbit抗原名称
Transferrin Receptor 2/TFR2分子别名
Transferrin receptor protein 2; TfR2免疫原
Synthetic Peptide细胞定位
Cell membrane, CytoplasmAccession
Q9UP52克隆号
S-1735-133抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu阳性样本
TF-1, HepG2, K562纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu IHC-P 1:250 Hu
Transferrin Receptor 2 (TFR2) is a single-pass type II membrane protein primarily expressed in the liver and bone marrow, playing a crucial role in iron homeostasis. Unlike its more widely studied counterpart, Transferrin Receptor 1 (TFR1), TFR2 is specifically involved in regulating iron uptake and signaling. It interacts with transferrin, a blood plasma protein that transports iron, and helps modulate the expression of hepcidin, the master regulator of iron absorption and distribution in the body. Mutations in the TFR2 gene are associated with hereditary hemochromatosis type III, a condition characterized by excessive iron accumulation. Additionally, TFR2 is involved in erythropoiesis, as it coordinates erythroid iron availability with erythropoietin receptor sensitivity. Its therapeutic potential lies in treating iron-related disorders, such as hereditary hemochromatosis and anemia of chronic disease, by modulating iron absorption and distribution.
免疫印迹
WB result of Transferrin Receptor 2/TFR2 Recombinant Rabbit mAb
Primary antibody: Transferrin Receptor 2/TFR2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: U-87 MG whole cell lysate 20 µg
Lane 2: TF-1 whole cell lysate 20 µg
Lane 3: HepG2 whole cell lysate 20 µg
Lane 4: K562 whole cell lysate 20 µg
Negative control: U-87 MG whole cell lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 89 kDa
Observed MW: 100 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human liver. Anti-Transferrin Receptor 2/TFR2 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human hepatocellular carcinoma. Anti-Transferrin Receptor 2/TFR2 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of Transferrin Receptor 2/TFR2 in human tissue.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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