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Rabbit抗原名称
SEC23B分子别名
Protein transport protein Sec23B; hSec23B; SEC23-related protein B免疫原
Synthetic Peptide细胞定位
Cytoplasm, Endoplasmic reticulumAccession
Q15437克隆号
S-1935-28抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt阳性样本
K562, Raji, MDA-MB-231, MCF7, SW620, RAW264.7, NIH/3T3, C6纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
ICC ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu, Ms, Rt IHC-P 1:250 Hu, Ms, Rt ICC 1:500 Hu
SEC23B, also known as Sec23 homolog B, is a crucial component of the coat protein complex II (COPII), which is involved in the transport of proteins from the endoplasmic reticulum (ER) to the Golgi apparatus. This gene plays a significant role in cellular processes such as autophagy regulation and cell migration. Recent studies have shown that SEC23B can induce autophagy in breast cancer cells by inhibiting the ERK/mTOR signaling pathway, thereby promoting cancer cell metastasis. Additionally, SEC23B has been implicated in various cancers, including thyroid cancer, hepatocellular carcinoma, and prostate cancer, suggesting its potential as a therapeutic target.
免疫印迹
WB result of SEC23B Recombinant Rabbit mAb
Primary antibody: SEC23B Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: K562 whole cell lysate 20 µg
Lane 2: Raji whole cell lysate 20 µg
Lane 3: MDA-MB-231 whole cell lysate 20 µg
Lane 4: MCF7 whole cell lysate 20 µg
Lane 5: SW620 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 86 kDa
Observed MW: 86 kDaWB result of SEC23B Recombinant Rabbit mAb
Primary antibody: SEC23B Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: RAW264.7 whole cell lysate 20 µg
Lane 2: NIH/3T3 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 86 kDa
Observed MW: 86 kDaWB result of SEC23B Recombinant Rabbit mAb
Primary antibody: SEC23B Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: C6 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 86 kDa
Observed MW: 86 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human tonsil. Anti- SEC23B antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human stomach. Anti- SEC23B antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human endometrial cancer. Anti- SEC23B antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human gastric cancer. Anti- SEC23B antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse colon. Anti- SEC23B antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat stomach. Anti- SEC23B antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
ICC shows positive staining in Raji cells. Anti- SEC23B antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
组织表达图谱
Expression of SEC23B in tumor tissue.
Expression of SEC23B in human tissue.
Expression of SEC23B in mouse & rat tissue.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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