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Rabbit抗原名称
Rab32分子别名
Ras-related protein Rab-32; RAB32免疫原
Synthetic Peptide细胞定位
MitochondrionAccession
Q13637克隆号
S-1936-49抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt阳性样本
HL-60, HeLa, HepG2, NIH/3T3, C6纯化方式
Protein A浓度
2 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu, Ms, Rt
Rab32 is a small GTPase protein belonging to the RAS oncogene family, involved in various cellular processes including mitochondrial dynamics, autophagy, and vesicle trafficking. It anchors the type II regulatory subunit of protein kinase A to the mitochondrial outer membrane, facilitating mitochondrial fission. Rab32 also interacts with LRRK2, a protein implicated in Parkinson's disease, and its variants have been identified as a susceptibility factor for familial Parkinson's disease. In cancer biology, Rab32 promotes glioblastoma migration and invasion by regulating ERK/Drp1-mediated mitochondrial fission. Additionally, it plays a role in the degradation of mitochondrial-proximal ER membranes through autophagy, mediated by its effector reticulon 3L.
免疫印迹
WB result of Rab32 Recombinant Rabbit mAb
Primary antibody: Rab32 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HL-60 whole cell lysate 20 µg
Lane 2: HeLa whole cell lysate 20 µg
Lane 3: HepG2 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 25 kDa
Observed MW: 25 kDaWB result of Rab32 Recombinant Rabbit mAb
Primary antibody: Rab32 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 25 kDa
Observed MW: 25 kDaWB result of Rab32 Recombinant Rabbit mAb
Primary antibody: Rab32 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: C6 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 25 kDa
Observed MW: 24 kDa
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变,不同客户在回收抗体的保存条件上也会有差异,所以抗体回收使用效果无法保证。另外,我们对一批抗体回收验证测试,测试结果显示不同抗体可回收次数不同,一般效价越高的抗体,可重复使用的次数越多,客户可根据实验情况来确定







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