PKR Recombinant Rabbit mAb (S-731-118)
Interferon-induced,double-stranded RNA-activated protein kinase,Eukaryotic translation initiation factor 2-alpha kinase 2 (eIF-2A protein kinase 2),Interferon-inducible RNA-dependent protein kinase,P1/eIF-2A protein kinase,Protein kinase RNA-activated (PKR,Protein kinase R),Tyrosine-protein kinase EIF2AK2,p68 kinase,PRKR,EIF2AK2
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宿主来源
Rabbit抗原名称
PKR分子别名
Interferon-induced, double-stranded RNA-activated protein kinase; Eukaryotic translation initiation factor 2-alpha kinase 2 (eIF-2A protein kinase 2); Interferon-inducible RNA-dependent protein kinase; P1/eIF-2A protein kinase; Protein kinase RNA-activated (PKR; Protein kinase R); Tyrosine-protein kinase EIF2AK2; p68 kinase; PRKR; EIF2AK2免疫原
Synthetic Peptide细胞定位
Nucleus, CytoplasmAccession
P19525克隆号
S-731-118抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu阳性样本
MCF7, HEK-293, K562, HeLa, HepG2, Jurkat纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
ICC ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu ICC 1:500 Hu
PKR, also known as Protein Kinase R or double-stranded RNA-dependent protein kinase, is a serine/threonine catalytic enzyme that plays a crucial role in innate immune responses against viral infections. It is an interferon-induced kinase that can be activated by double-stranded RNA (dsRNA), a common byproduct of viral replication. Once activated, PKR phosphorylates the eukaryotic initiation factor 2α (eIF2α), which inhibits protein synthesis and viral replication. Additionally, PKR can activate the NF-κB signaling pathway, enhancing the expression of antiviral genes. Beyond its antiviral functions, PKR is involved in various cellular processes, including apoptosis, cell growth, and differentiation. Its dysregulation has been implicated in several diseases, including cancer, making it a potential therapeutic target.
免疫印迹
WB result of PKR Recombinant Rabbit mAb
Primary antibody: PKR Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: MCF7 whole cell lysate 20 µg
Lane 2: HEK-293 whole cell lysate 20 µg
Lane 3: K562 whole cell lysate 20 µg
Lane 4: HeLa whole cell lysate 20 µg
Lane 5: HepG2 whole cell lysate 20 µg
Lane 6: Jurkat whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 62 kDa
Observed MW: 38, 70 kDa
免疫细胞化学
ICC shows positive staining in MCF7 cells. Anti- PKR antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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