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Rabbit抗原名称
Phospho-PKCδ (Thr505)分子别名
Protein kinase C delta type; Tyrosine-protein kinase PRKCD; nPKC-delta; PKCD; PRKCD免疫原
Synthetic Peptide细胞定位
Nucleus, Mitochondrion, Cell membrane, CytoplasmAccession
Q05655克隆号
S-1668-89抗体类型
Recombinant mAb抗体同种型
IgG翻译后修饰类型
磷酸化反应种属 ?
Hu阳性样本
THP-1纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
WB
稀释度
应用 稀释度 推荐种属 Dot Blot 1:1000 WB 1:1000 Hu
Phospho-PKCδ (Thr505) refers to the phosphorylation of protein kinase C delta (PKCδ) at threonine residue 505, a modification that is crucial for its activation and function. PKCδ is a member of the protein kinase C (PKC) family, which are serine/threonine kinases involved in various cellular signaling pathways, including regulation of cell growth, apoptosis, and differentiation. The phosphorylation at Thr505 is particularly important for the kinase activity of PKCδ and its role in the G1 to S phase transition during cell cycle progression. This phosphorylation site is specific to PKCδ, and antibodies targeting Phospho-PKCδ (Thr505) do not cross-react with other PKC isoforms, making it a valuable marker for studying PKCδ's unique functions.
免疫印迹
WB result of Phospho-PKCδ (Thr505) Recombinant Rabbit mAb
Primary antibody: Phospho-PKCδ (Thr505) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated THP-1 whole cell lysate 20 µg
Lane 2: THP-1 treated with 0.05 µM PMA for 12 hours whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 78 kDa
Observed MW: 78 kDa
斑点杂交
Dot blot result of Phospho-PKCδ (Thr505) Recombinant Rabbit mAb
Lane 1: PKC δ (Thr505) phospho peptide
Lane 2: PKC δ (Thr505) unmodified peptide
Primary antibody: Phospho-PKCδ (Thr505) Recombinant Rabbit mAb at 1/1000 dilution
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
我们推荐客户使用TPST+5%脱脂奶粉来稀释一抗，进行封闭。 虽然BSA被推荐为WB检测磷酸化蛋白的常用封闭剂，但是脱脂奶粉获取更加方便，覆盖更广泛的非特异性结合位点，在一抗性能优越的前提下，使用脱脂奶粉封闭性价比更高
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