MICB Recombinant Rabbit mAb (S-1472-102)

MHC class I polypeptide-related sequence B,MIC-B,PERB11.2

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B1426

规格

25μl100μl1ml

数量

产品介绍

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产品规格

应用	稀释度	推荐种属
WB	1:1000	Hu
ICC	1:500	Hu

背景介绍

MICB (MHC class I polypeptide-related sequence B) is a stress-inducible, heavily glycosylated transmembrane protein encoded by the MICB gene located on chromosome 6p21.33. It functions as a ligand for the NKG2D receptor, which is expressed on natural killer (NK) cells, CD8+ T cells, and γδ T cells. Upon binding, MICB activates the cytolytic response of these immune cells, promoting their ability to target and eliminate stressed or transformed cells, such as tumor cells or virus-infected cells. Unlike classical MHC class I molecules, MICB does not associate with β-2-microglobulin or bind peptides. Its expression is induced by cellular stress, including DNA damage and viral infections, making it a crucial component in both innate and adaptive immune responses. MICB is widely expressed in various tissues, including lymph nodes and bone marrow, highlighting its role in diverse physiological contexts.

免疫印迹
- WB result of MICB Recombinant Rabbit mAb
  Primary antibody: MICB Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: Purified human MICB recombinant protein 10 ng
  Lane 2: Purified human MICA recombinant protein 10 ng
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 43 kDa
  Observed MW: 37-50 kDa
- WB result of MICB Recombinant Rabbit mAb
  Primary antibody: MICB Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: THP-1 whole cell lysate 20 µg
  Lane 2: T-47D whole cell lysate 20 µg
  Lane 3: K562 whole cell lysate 20 µg
  Lane 4: K562 treated with RNGase F whole cell lysate 20 µg
  Lane 5: HCT 116 whole cell lysate 20 µg
  Negative control: THP-1 whole cell lysate
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 43 kDa
  Observed MW: 40-70 kDa
免疫细胞化学
- ICC shows positive staining in K562 cells (top panel) and negative staining in THP-1 cells (below panel). Anti-MICB antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).