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Rabbit抗原名称
Integrin beta 4分子别名
Integrin beta-4; CD104; Itgb4免疫原
Recombinant Protein细胞定位
Cell membraneAccession
A2A863克隆号
S-1625-170抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Ms阳性样本
mouse spleen, mouse pancreas纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
IF ?
稀释度
应用 稀释度 推荐种属 WB 1:1000 Ms IHC-P 1:200 Ms IF 1:100 Ms
Integrin beta 4 (ITGB4) is a key transmembrane receptor that primarily forms heterodimers with integrin alpha 6 to mediate cell adhesion and signaling, playing crucial roles in maintaining the integrity of epithelial tissues. It is predominantly expressed in epithelial cells and localized at the basement membrane zone, where it contributes to the formation and stabilization of hemidesmosomes, specialized structures that anchor cells to the extracellular matrix. ITGB4 is involved in various biological processes, including cell migration, proliferation, and survival, and its dysregulation has been implicated in several diseases, such as junctional epidermolysis bullosa, a genetic disorder characterized by severe skin blistering. Additionally, ITGB4 has been found to promote tumorigenesis and cancer cell invasion by interacting with other signaling pathways, such as receptor tyrosine kinases, and facilitating epithelial-mesenchymal transition in various cancers.
免疫印迹
WB result of Integrin beta 4 Recombinant Rabbit mAb
Primary antibody: Integrin beta 4 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse spleen lysate 20 µg
Lane 2: mouse pancreas lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 202 kDa
Observed MW: 250 kDa
免疫组化
IHC shows positive staining in paraffin-embedded mouse colon. Anti-Integrin beta 4 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse pancreas. Anti-Integrin beta 4 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse stomach. Anti-Integrin beta 4 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫荧光
IF shows positive staining in paraffin-embedded mouse colon. Anti- Integrin beta 4 antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded mouse spleen. Anti- Integrin beta 4 antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
组织表达图谱
Expression of Integrin beta 4 in mouse tissue.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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