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产品中心 蛋白翻译后修饰(PTM)抗体 位点特异性修饰抗体

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Histone H3 (citrulline R2 + R8 + R17) Recombinant Rabbit mAb (S-1733-17)

Histone H3.1,Histone H3/a,Histone H3/b,Histone H3/c,Histone H3/d,Histone H3/f

价格 ￥600.00 （ 供应商现货 ： 3-5个工作日）
货号 S0B1423
规格
数量
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产品介绍

产品介绍 FAQs 评论(0)

产品规格

  • 宿主来源

    Rabbit

  • 抗原名称

    Histone H3 (citrulline R2 + R8 + R17)

  • 分子别名

    Histone H3.1; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f

  • 免疫原

    Synthetic Peptide

  • 细胞定位

    Nucleus

  • Accession

    P68431

  • 克隆号

    S-1733-17

  • 抗体类型

    Recombinant mAb

  • 抗体同种型

    IgG

  • 翻译后修饰类型

    瓜氨酸化

  • 反应种属 ?

    Hu

  • 纯化方式

    Protein A

  • 浓度

    0.5 mg/ml

  • 标记

    Unconjugated

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

  • 应用

    WB

  • 稀释度

    应用 稀释度 推荐种属
    Dot Blot 1:1000
    WB 1:1000 Hu
背景介绍

  • Histone H3 is a core component of the nucleosome, the fundamental unit of chromatin, which plays a crucial role in organizing genomic DNA within the eukaryotic nucleus. The citrullination of Histone H3 at arginine residues R2, R8, and R17 is a specific post-translational modification catalyzed by peptidyl arginine deiminase 4 (PAD4). This modification prevents methylation of these residues by protein-arginine methyltransferase 4 (PRMT4) and is associated with transcriptional repression. Citrullinated Histone H3 is also a key component of neutrophil extracellular traps (NETs) and acts as an autoantigen, contributing to the production of anti-citrullinated protein antibodies in various diseases, including sepsis, multiple sclerosis, rheumatoid arthritis, and multiple myeloma.

  • 免疫印迹

    • WB result of Histone H3 (citrulline R2 + R8 + R17) Recombinant Rabbit mAb
      Primary antibody: Histone H3 (citrulline R2 + R8 + R17) Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: untreated HEK-293 whole cell lysate 20 µg
      Lane 2: HEK-293 transfected with PADI4 expression vector, then treated with 10 mM CaCl2 and 10 µM Ionomycin for 2 hours whole cell lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 15 kDa
      Observed MW: 17 kDa

  • 斑点杂交

    • Dot blot result of Histone H3 (citrulline R2 + R8 + R17) Recombinant Rabbit mAb
      Lane 1: Histone H3 (citrulline R2+R8+R17) modified peptide
      Lane 2: Histone H3 (citrulline R2) modified peptide
      Lane 3: Histone H3 (citrulline R8) modified peptide
      Lane 4: Histone H3 (citrulline R17) modified peptide
      Lane 5: Histone H3 (citrulline R2+R8+R17) unmodified peptide
      Primary antibody: Histone H3 (citrulline R2 + R8 + R17) Recombinant Rabbit mAb at 1/1000 dilution
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution

FAQs

斯达特公司的抗体，可以回收利用几次？

我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定

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