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Rabbit抗原名称
FCER1A/FcεRIα分子别名
High affinity immunoglobulin epsilon receptor subunit alpha; Fc-epsilon RI-alpha (FcERI); IgE Fc receptor subunit alpha; FCE1A免疫原
Recombinant Protein细胞定位
Cell membraneAccession
P12319克隆号
S-1697-174抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu阳性样本
KU812纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
ICC ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu IHC-P 1:50 Hu ICC 1:500 Hu
FcεRIα (Fc epsilon receptor I alpha) is a type I transmembrane glycoprotein encoded by the FCER1A gene, which belongs to the immunoglobulin gene superfamily. It is a critical component of the high-affinity IgE receptor (FcεRI) complex, responsible for binding the Fc region of IgE antibodies with high affinity. This receptor complex is primarily expressed on mast cells and basophils, and to a lesser extent on some monocytes, Langerhans cells, dendritic cells, and eosinophils from allergic donors. Upon allergen binding to IgE, FcεRIα triggers cellular activation, leading to the release of mediators such as histamine and cytokines, which drive allergic responses and contribute to anti-parasite immunity.
免疫印迹
WB result of FCER1A/FcεRIα Recombinant Rabbit mAb
Primary antibody: FCER1A/FcεRIα Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HEK-293 whole cell lysate 20 µg
Lane 2: KU812 whole cell lysate 20 µg
Negative control: HEK-293 whole cell lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 30 kDa
Observed MW: 48 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human prostatic cancer. Anti-FCER1A/FcεRIα antibody was used at 1/50 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human renal clear cell carcinoma. Anti-FCER1A/FcεRIα antibody was used at 1/50 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human tonsil. Anti-FCER1A/FcεRIα antibody was used at 1/50 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast cancer. Anti-FCER1A/FcεRIα antibody was used at 1/50 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cervical squamous cell carcinoma. Anti-FCER1A/FcεRIα antibody was used at 1/50 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
ICC shows positive staining in KU812 cells (top panel) and negative staining in 293T cells (below panel). Anti- FCER1A/FcεRIα antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
组织表达图谱
Expression of FCER1A/FcεRIα in tumor tissue.
Expression of FCER1A/FcεRIα in human tissue.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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