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Rabbit抗原名称
CXCL10/IP-10分子别名
C-X-C motif chemokine 10; 10 kDa interferon gamma-induced protein (Gamma-IP10; IP-10); Small-inducible cytokine B10; INP10; SCYB10免疫原
Recombinant Protein细胞定位
SecretedAccession
P02778克隆号
S-1910-59抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu
CXCL10, also known as interferon γ-induced protein 10 kDa (IP-10), is a cytokine belonging to the CXC chemokine family. It exerts its biological effects by binding to the CXCR3 receptor, which is predominantly expressed on activated T cells, B cells, natural killer (NK) cells, dendritic cells, and macrophages. CXCL10 is a pleiotropic molecule with diverse functions, including promoting the chemotaxis of CXCR3+ cells, inducing apoptosis, regulating cell growth and proliferation, and modulating angiogenesis in infectious, inflammatory, and cancerous conditions. It is secreted by various cell types, such as leukocytes, epithelial cells, and fibroblasts, in response to IFN-γ stimulation. The CXCL10 gene was first isolated in 1985 and encodes a 12 kDa protein with two internal disulfide cross-bridges. Its expression levels are associated with immune responses, tissue damage, and tumor formation, making it a significant factor in both innate and adaptive immunity.
免疫印迹
WB result of CXCL10/IP-10 Recombinant Rabbit mAb
Primary antibody: CXCL10/IP-10 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated THP-1 whole cell lysate 20 µg
Lane 2: THP-1 treated with 100 ng/ml PMA for 24 hours, then 10 pg/ml LPS for 24 hours and 20 ng/ml IFN-γ for 24 hours, and 300 ng/ml Brefeldin A for the last 4 hours whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 11 kDa
Observed MW: 12 kDa
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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