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c-Jun Recombinant Rabbit mAb (S-494-457)

Transcription factor Jun,Activator protein 1 (AP1),Proto-oncogene c-Jun,Transcription factor AP-1 subunit Jun,V-jun avian sarcoma virus 17 oncogene homolog,p39,JUN

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B1420

规格

25μl100μl1ml

数量

产品介绍

产品介绍 FAQs 评论(0)

产品规格

宿主来源

Rabbit
抗原名称

c-Jun
分子别名

Transcription factor Jun; Activator protein 1 (AP1); Proto-oncogene c-Jun; Transcription factor AP-1 subunit Jun; V-jun avian sarcoma virus 17 oncogene homolog; p39; JUN
免疫原

Synthetic Peptide
细胞定位

Nucleus
Accession

P05412
克隆号

S-494-457
抗体类型

Recombinant mAb
抗体同种型

IgG
反应种属 ?

Hu, Ms
阳性样本

PC-3, MDA-MB-231
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

ChIP

WB
稀释度

应用稀释度推荐种属

WB 1:1000 Hu, MS
ChIP 1:20-1:50 Hu

应用	稀释度	推荐种属
WB	1:1000	Hu, MS
ChIP	1:20-1:50	Hu

背景介绍

c-Jun is a key component of the transcription factor complex AP-1 (Activator Protein 1) and is one of the most transcriptionally active members of this complex. It is involved in a wide range of cellular processes, including cell proliferation, differentiation, apoptosis, and the regulation of inflammatory responses. c-Jun can be activated by various stimuli, such as growth factors, cytokines, and stress signals, which lead to its phosphorylation and subsequent regulation of gene expression. In cancer biology, c-Jun has been implicated in tumor initiation and progression, particularly in liver cancer, where it can promote cell survival and inhibit apoptosis through interactions with other signaling pathways. Additionally, c-Jun plays a role in immune cell function and has been studied for its impact on T-cell exhaustion and immune response regulation. Its diverse functions highlight its importance as a regulatory hub in cellular signaling and gene expression networks.

免疫印迹
- WB result of c-Jun Recombinant Rabbit mAb
  Primary antibody: c-Jun Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: T-47D whole cell lysate 20 µg
  Lane 2: PC-3 whole cell lysate 20 µg
  Lane 3: MDA-MB-231 whole cell lysate 20 µg
  Negative control: T-47D whole cell lysate
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 36 kDa
  Observed MW: 38 kDa
- WB result of c-Jun Recombinant Rabbit mAb
  Primary antibody: c-Jun Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: NIH/3T3 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 36 kDa
  Observed MW: 38 kDa
ChIP
- Chromatin immunoprecipitation (ChIP) was performed on PC-3 cells cross-linked with 1% formaldehyde for 10 min, then chromatin was fragmented by sonication. Parallel reactions used c-Jun Recombinant Rabbit mAb (S-494-457) and Rabbit mAb IgG Isotype Control (SDT-R173) at 1:50 for immunoprecipitation.
  Post - immunoprecipitation, both samples were washed, eluted, and cross-links reversed. Purified DNA was analyzed by qPCR.
  qPCR showed the enrichment of NRIPI and SAT-α in c-Jun Recombinant Rabbit
  mAb (S-494-457)-immunoprecipitated sample.