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宿主来源
Rabbit抗原名称
ZO-1分子别名
TJP1; tight junction protein 1细胞定位
Cell membrane, Cell junctionAccession
Q07157抗体类型
Recombinant mAb抗体同种型
IgG应用
IHC-P反应种属 ?
Hu纯化方式
Protein A标记
Unconjugated性状
Liquid缓冲体系
50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
| 应用 | 稀释度 |
|---|---|
| IHC-P | 1:100-1:200 |
ZO-1 (zonula occludens-1) protein is a member of the membrane-associated guanylate kinase (MAGUK) family and a key component of tight junctions, which are crucial for maintaining the integrity and permeability of epithelial and endothelial barriers. It contains three PDZ domains, a SH3 domain, and a guanylate kinase (GUK) domain, allowing it to interact with various transmembrane proteins like claudins, JAM-A, and occludin, as well as cytoplasmic proteins such as cortactin and α-catenin. ZO-1 plays a vital role in organizing the cytoskeleton, particularly in endothelial cells, where it controls barrier function, cell migration, and angiogenesis. It also participates in signaling pathways, such as the Hippo-independent YAP/TAZ pathway, influencing cell proliferation and transcriptional regulation. Additionally, ZO-1 interacts with YB-1 to regulate stress granule formation in endothelial cells during angiogenesis, providing cytoprotection against stress.
免疫组化
IHC shows positive staining in paraffin-embedded human heart muscle. Anti-ZO-1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human stomach. Anti-ZO-1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human kidney. Anti-ZO-1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human liver cancer. Anti-ZO-1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung adenocarcinoma. Anti-ZO-1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
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