所有图片（1/12）

Scavenging Receptor SR-BI Recombinant Rabbit mAb (S-1736-15)

Scavenger receptor class B member 1,SRB1,CD36 and LIMPII analogous 1 (CLA-1),CD36 antigen-like 1,Collagen type I receptor,thrombospondin receptor-like 1,SR-BI,CD36,CD36L1,CLA1,SCARB1

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B1412

规格

25μl100μl1ml

数量

产品介绍

产品介绍 FAQs 评论(0)

产品规格

宿主来源

Rabbit
抗原名称

Scavenging Receptor SR-BI
分子别名

Scavenger receptor class B member 1; SRB1; CD36 and LIMPII analogous 1 (CLA-1); CD36 antigen-like 1; Collagen type I receptor; thrombospondin receptor-like 1; SR-BI; CD36; CD36L1; CLA1; SCARB1
免疫原

Recombinant Protein
细胞定位

Cell membrane
Accession

Q8WTV0
克隆号

S-1736-15
抗体类型

Recombinant mAb
抗体同种型

IgG
反应种属 ?

Hu, Ms, Rt
阳性样本

HepG2, THP-1, NIH/3T3, rat liver
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

IHC-P ?

ICC ?

WB
稀释度

应用稀释度推荐种属

WB 1:1000-1:5000 Hu, Ms, Rt
IHC-P 1:1000 Hu, Ms, Rt
ICC 1:500 Hu

应用	稀释度	推荐种属
WB	1:1000-1:5000	Hu, Ms, Rt
IHC-P	1:1000	Hu, Ms, Rt
ICC	1:500	Hu

背景介绍

Scavenger receptor class B type I (SR-BI) is a versatile and physiologically significant HDL receptor that plays a crucial role in cholesterol homeostasis and lipoprotein metabolism. It is a cell surface glycoprotein highly expressed in the liver, steroidogenic tissues, endothelium, and other tissues. SR-BI mediates the selective uptake of cholesterol esters from HDL particles into cells through hydrophobic channels without internalizing the entire lipoprotein, facilitating reverse cholesterol transport. This process helps remove excess cholesterol from peripheral tissues and promotes its excretion via bile acids, thus exerting anti-atherosclerotic effects. Additionally, SR-BI is involved in various biological functions, including regulation of inflammation, endothelial cell function, and lipid metabolism in macrophages. Its dysfunction or genetic mutations are associated with increased cardiovascular risk and altered HDL metabolism.

免疫印迹
- WB result of Scavenging Receptor SR-BI Recombinant Rabbit mAb
  Primary antibody: Scavenging Receptor SR-BI Recombinant Rabbit mAb at 1/5000 dilution
  Lane 1: HepG2 whole cell lysate 20 µg
  Lane 2: THP-1 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 61 kDa
  Observed MW: 70 kDa
- WB result of Scavenging Receptor SR-BI Recombinant Rabbit mAb
  Primary antibody: Scavenging Receptor SR-BI Recombinant Rabbit mAb at 1/5000 dilution
  Lane 1: NIH/3T3 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 61 kDa
  Observed MW: 70 kDa
- WB result of Scavenging Receptor SR-BI Recombinant Rabbit mAb
  Primary antibody: Scavenging Receptor SR-BI Recombinant Rabbit mAb at 1/5000 dilution
  Lane 1: rat liver lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 61 kDa
  Observed MW: 90 kDa
免疫组化
- IHC shows positive staining in paraffin-embedded human liver. Anti-Scavenging Receptor SR-BI antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human breast cancer. Anti-Scavenging Receptor SR-BI antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human diffuse large B-cell lymphoma. Anti-Scavenging Receptor SR-BI antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded mouse liver. Anti-Scavenging Receptor SR-BI antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded rat liver. Anti-Scavenging Receptor SR-BI antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
- ICC shows positive staining in HepG2 cells. Anti-Scavenging Receptor SR-BI antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
组织表达图谱
- Expression of Scavenging Receptor SR-BI in tumor tissue.
- Expression of Scavenging Receptor SR-BI in human tissue.
- Expression of Scavenging Receptor SR-BI in mouse & rat tissue.