Phospho-Stat3 (Tyr705) Recombinant Rabbit mAb (S-1829-143)

Signal transducer and activator of transcription 3,Acute-phase response factor,APRF,STAT3

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B1411

规格

25μl100μl1ml

数量

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产品规格

宿主来源

Rabbit
抗原名称

Phospho-Stat3 (Tyr705)
分子别名

Signal transducer and activator of transcription 3; Acute-phase response factor; APRF; STAT3
免疫原

Synthetic Peptide
细胞定位

Nucleus
Accession

P40763
克隆号

S-1829-143
抗体类型

Recombinant mAb
抗体同种型

IgG
翻译后修饰类型

磷酸化
应用

ChIP ,
WB ,
IP
反应种属 ?

Hu, Ms, Rt
预测反应种属
(反应种属缩写表)

Bv, Pg
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied

稀释度

应用	稀释度	推荐种属
Dot Blot	1:1000
WB	1:1000	Hu
IP	1:50	Hu
ChIP	1:20-1:50	Hu

背景介绍

Phospho-Stat3 (Tyr705) is a critical biomarker indicating the activation of the STAT3 protein, a latent cytoplasmic transcription factor involved in various cellular processes such as proliferation, survival, and immune response. When phosphorylated at tyrosine 705, STAT3 undergoes dimerization and translocates to the nucleus, where it binds to DNA and regulates gene expression. This phosphorylation is primarily mediated by the JAK family of receptor-associated tyrosine kinases, particularly JAK1, in response to cytokine signaling. Phospho-Stat3 (Tyr705) is also implicated in diseases like cancer, where its constitutive activation is observed in various tumor types.

免疫印迹
- WB result of Phospho-Stat3 (Tyr705) Recombinant Rabbit mAb
  Primary antibody: Phospho-Stat3 (Tyr705) Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: untreated HeLa whole cell lysate 20 µg
  Lane 2: HeLa treated with 50 ng/ml IFN-α for 30 minutes whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 88 kDa
  Observed MW: 80, 90 kDa
免疫沉淀
- Phospho-Stat3 (Tyr705) Recombinant Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating Phospho-Stat3 (Tyr705) in 0.4 mg HeLa + IFN-α(50 ng/ml, 30 min) whole cell lysate.
  Western blot was performed on the immunoprecipitate using Phospho-Stat3 (Tyr705) Recombinant Rabbit mAb at 1/1000 dilution.
  Secondary antibody (HRP) for IP was used at 1/1000 dilution.
  Lane 1: HeLa+IFN-α(50 ng/ml, 30 min) whole cell lysate 20 µg (Input)
  Lane 2: Phospho-Stat3 (Tyr705) Recombinant Rabbit mAb IP in HeLa + IFN-α(50 ng/ml, 30 min) whole cell lysate
  Lane 3: Rabbit monoclonal IgG IP in HeLa + IFN-α(50 ng/ml, 30 min) whole cell lysate
  Predicted MW: 88 kDa
  Observed MW: 80, 90 kDa
斑点杂交
- Dot blot result of Phospho-Stat3 (Tyr705) Recombinant Rabbit mAb
  Lane 1: Stat3 (Tyr705) phospho peptide
  Lane 2: Stat3 unmodified peptide
  Primary antibody: Phospho-Stat3 (Tyr705) Recombinant Rabbit mAb at 1/1000 dilution
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
ChIP
- Chromatin immunoprecipitation (ChIP) was performed on HeLa + IFN-α (50 ng/ml, 30 min) cells cross - linked with 1% formaldehyde for 10 min, then chromatin was fragmented by sonication. Parallel reactions used Phospho Stat3 (Tyr705) Recombinant Rabbit mAb (S-1829-143) and Rabbit mAb IgG Isotype Control (SDT-R173) at 1:50 for immunoprecipitation. Post - immunoprecipitation, both samples were washed, eluted, and cross - links reversed. Purified DNA was analyzed by qPCR.
  qPCR showed the enrichment of c-FOS, IRF1 and SAT-α in Phospho-Stat3 (Tyr705)
  Recombinant Rabbit mAb (S-1829-143)-immunoprecipitated sample.