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Rabbit抗原名称
Phospho-Histone H3 (Ser10)分子别名
H3S10P免疫原
Synthetic Peptide细胞定位
NucleusAccession
P68431克隆号
S-960-58抗体类型
Recombinant mAb抗体同种型
IgG翻译后修饰类型
磷酸化反应种属 ?
Hu, Ms, Rt预测反应种属
(反应种属缩写表)Ar, Bv, C.el, Dr, Hm, Or, Xe, Ys纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
ChIP
WB
稀释度
应用 稀释度 推荐种属 Dot Blot 1:10000 WB 1:20000 Hu, Ms, Rt ChIP 1:20-1:50 Hu, Ms, Rt
Phospho-Histone H3 (Ser10) is a critical epigenetic marker associated with chromosome condensation and mitotic progression. This phosphorylation event typically occurs during the G2 to M phase transition and is essential for chromatin condensation, making it a specific indicator of mitotic cells. It is regulated by kinases such as Aurora B and plays a significant role in mitotic chromosome condensation and decondensation. Additionally, H3 Ser10 phosphorylation has been implicated in transcriptional regulation and early gene expression, and its dysregulation has been linked to neoplastic cell transformation and cancer.
免疫印迹
WB result of Phospho-Histone H3 (Ser10) Recombinant Rabbit mAb
Primary antibody: Phospho-Histone H3 (Ser10) Recombinant Rabbit mAb at 1/20000 dilution
Lane 1: untreated HeLa whole cell lysate 20 µg
Lane 2: HeLa treated with 100 ng/ml Nocodazole for 18 hours and 100 nM Calyculin A for 1 hour whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 15 kDa
Observed MW: 17 kDaWB result of Phospho-Histone H3 (Ser10) Recombinant Rabbit mAb
Primary antibody: Phospho-Histone H3 (Ser10) Recombinant Rabbit mAb at 1/20000 dilution
Lane 1: HeLa treated with 100 ng/ml Nocodazole for 18 hours and 100 nM Calyculin A for 1 hour whole cell lysate 20 µg
Lane 2: HeLa treated with 100 ng/ml Nocodazole for 18 hours and 100 nM Calyculin A for 1 hours, then treated with phosphatase whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 15 kDa
Observed MW: 17 kDaWB result of Phospho-Histone H3 (Ser10) Recombinant Rabbit mAb
Primary antibody: Phospho-Histone H3 (Ser10) Recombinant Rabbit mAb at 1/20000 dilution
Lane 1: untreated NIH/3T3 whole cell lysate 20 µg
Lane 2: NIH/3T3 strave 3 hours, then treated with 100 nM Calyculin A for 30 minutes whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 15 kDa
Observed MW: 17 kDaWB result of Phospho-Histone H3 (Ser10) Recombinant Rabbit mAb
Primary antibody: Phospho-Histone H3 (Ser10) Recombinant Rabbit mAb at 1/20000 dilution
Lane 1: untreated C6 whole cell lysate 20 µg
Lane 2: C6 treated with 100 nM Calyculin A for 30 minutes whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 15 kDa
Observed MW: 17 kDa
斑点杂交
Dot blot result of Phospho-Histone H3 (Ser10) Recombinant Rabbit mAb
Lane 1: Histone H3 phospho S10 peptide
Lane 2: Histone H3 phospho S10 peptide
Lane 3: Histone H3 unmodified peptide
Primary antibody: Phospho-Histone H3 (Ser10) Recombinant Rabbit mAb at 1/10000 dilution
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
ChIP
Chromatin immunoprecipitation (ChIP) was performed on HeLa + Nocodazole (100ng/ml,18h) (+) (left) and HeLa + Nocodazole (100ng/ml,18h) (-) (right) cells
cross - linked with 1% formaldehyde for 10 min, then chromatin was fragmented by sonication. Parallel reactions used Phospho-Histone H3 (Ser10) Recombinant Rabbit mAb (S-960-58) and Rabbit mAb IgG
Isotype Control (SDT-R173) at 1:50 for immunoprecipitation.
Post - immunoprecipitation, both samples were washed, eluted, and cross - links reversed. Purified DNA was analyzed by qPCR.
qPCR (%input: immunoprecipitated DNA / input DNA)
showed the enrichment of RPL30 and GAPDH in Phospho-
Histone H3(Ser10) Recombinant Rabbit mAb (S-960-58)-
immunoprecipitated sample.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
我们推荐客户使用TPST+5%脱脂奶粉来稀释一抗，进行封闭。 虽然BSA被推荐为WB检测磷酸化蛋白的常用封闭剂，但是脱脂奶粉获取更加方便，覆盖更广泛的非特异性结合位点，在一抗性能优越的前提下，使用脱脂奶粉封闭性价比更高
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