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Rabbit抗原名称
SMAD2分子别名
Mothers against decapentaplegic homolog 2; MAD homolog 2; Mothers against DPP homolog 2; JV18-1; Mad-related protein 2 (hMAD-2); SMAD family member 2 (SMAD 2; Smad2; hSMAD2); MADH2; MADR2免疫原
Recombinant Protein细胞定位
Nucleus, CytoplasmAccession
Q15796克隆号
S-1521-177抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt阳性样本
HeLa, HCT 116, HEK-293, mouse brain, C6, rat brain预测反应种属
(反应种属缩写表)Bv纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu, Ms, Rt IHC-P 1:200 Hu, Ms, Rt ICC 1:500 Hu ICFCM 1:50 Hu ChIP 1:20 Hu
SMAD2 is a key protein in the TGF-β signaling pathway, which plays a crucial role in regulating various cellular processes such as cell growth, differentiation, and immune responses. When TGF-β binds to its receptors, it activates the receptor complex, leading to the phosphorylation of SMAD2. Phosphorylated SMAD2 then forms a complex with SMAD4 and other cofactors, acting as a transcription factor to regulate gene expression. This pathway is essential for maintaining cellular homeostasis, but dysregulation of SMAD2 and TGF-β signaling has been implicated in diseases such as fibrosis, cancer, and atherosclerosis.
免疫印迹
WB result of SMAD2 Recombinant Rabbit mAb
Primary antibody: SMAD2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: HCT 116 whole cell lysate 20 µg
Lane 3: HEK-293 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 52 kDa
Observed MW: 58 kDaWB result of SMAD2 Recombinant Rabbit mAb
Primary antibody: SMAD2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse brain lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 52 kDa
Observed MW: 58 kDaWB result of SMAD2 Recombinant Rabbit mAb
Primary antibody: SMAD2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: C6 whole cell lysate 20 µg
Lane 2: rat brain lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 52 kDa
Observed MW: 58 kDa
流式分析
Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized MCF7 (Human breast adenocarcinoma epithelial cell) labelling SMAD2 antibody at 1/50 dilution (1 μg) / (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
免疫组化
IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-SMAD2 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse kidney. Anti-SMAD2 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat kidney. Anti-SMAD2 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
ICC shows positive staining in MCF7 cells. Anti- SMAD2 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
ChIP
Chromatin immunoprecipitation (ChIP) was performed on HaCaT + TGF-β (7ng/mL, 1h) cells cross - linked with 1% formaldehyde for 10 min, then chromatin was fragmented by sonication. Parallel reactions used SMAD2 Recombinant Rabbit mAb (S-1521-177) and Rabbit mAb IgG Isotype Control (SDT-R173) at 1:20 for immunoprecipitation.
Post - immunoprecipitation, both samples were washed, eluted, and cross - links reversed. Purified DNA was analyzed by qPCR.
qPCR showed the enrichment of ID1, CDKN1A and SAT-α in SMAD2 Recombinant Rabbit mAb (S-1521-177)-immunoprecipitated sample.
组织表达图谱
Expression of SMAD2 in tumor tissue.
Expression of SMAD2 in human tissue.
Expression of SMAD2 in mouse & rat tissue.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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