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Rabbit抗原名称
CHMP6/VPS20分子别名
Charged multivesicular body protein 6; Chromatin-modifying protein 6; Vacuolar protein sorting-associated protein 20 (Vps20; hVps20)免疫原
Synthetic Peptide细胞定位
EndosomeAccession
Q96FZ7克隆号
S-1702-78抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Ms, Rt阳性样本
mouse testis, mouse lung, rat lung预测反应种属
(反应种属缩写表)Gor纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Ms, Rt IHC-P 1:250 Ms, Rt
CHMP6, also known as VPS20, is a protein encoded by the human gene CHMP6 and belongs to the charged multivesicular body protein family. It is a core component of the ESCRT-III (endosomal sorting complex required for transport III) complex, which is essential for the formation of multivesicular bodies (MVBs) and the sorting of endosomal cargo proteins into these MVBs. MVBs are involved in the degradation of membrane proteins, such as growth factor receptors, lysosomal enzymes, and lipids, by transporting them to lysosomes. CHMP6 is also known to interact with other components of the ESCRT machinery, such as CHMP4 and members of the ESCRT-II complex, to regulate cargo sorting and membrane remodeling processes. The protein is highly conserved across species and contains specific domains like coiled-coil structures and an N-myristoylation site, which are crucial for its function.
免疫印迹
WB result of CHMP6/VPS20 Recombinant Rabbit mAb
Primary antibody: CHMP6/VPS20 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse testis lysate 20 µg
Lane 2: mouse lung lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 23 kDa
Observed MW: 27 kDaWB result of CHMP6/VPS20 Recombinant Rabbit mAb
Primary antibody: CHMP6/VPS20 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat lung lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 23 kDa
Observed MW: 25 kDa
免疫组化
IHC shows positive staining in paraffin-embedded mouse colon. Anti-CHMP6/VPS20 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse liver. Anti-CHMP6/VPS20 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat testis. Anti-CHMP6/VPS20 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of CHMP6/VPS20 in mouse & rat tissue.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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