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eNOS Recombinant Rabbit mAb (S-1885-94)

Nitric oxide synthase 3,Constitutive NOS (cNOS),EC-NOS,NOS type III (NOSIII),Nitric oxide synthase,endothelial (Endothelial NOS,eNOS),NOS3

价格 ￥600.00 （ 供应商现货 ： 3-5个工作日）
货号 S0B1404
规格
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产品规格

  • 宿主来源

    Rabbit

  • 抗原名称

    eNOS

  • 分子别名

    Nitric oxide synthase 3; Constitutive NOS (cNOS); EC-NOS; NOS type III (NOSIII); Nitric oxide synthase; endothelial (Endothelial NOS; eNOS); NOS3

  • 免疫原

    Synthetic Peptide

  • 细胞定位

    Cytoplasm, Cell membrane

  • Accession

    P29474

  • 克隆号

    S-1885-94

  • 抗体类型

    Recombinant mAb

  • 抗体同种型

    IgG

  • 应用

    IHC-P, WB

  • 反应种属 ?

    Hu, Ms, Rt

  • 阳性样本

    HUVEC, mouse liver, mouse spleen, rat liver

  • 纯化方式

    Protein A

  • 浓度

    0.5 mg/ml

  • 标记

    Unconjugated

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

稀释度
应用 稀释度 推荐种属
WB 1:1000 Hu, Ms, Rt
IHC-P 1:250 Hu, Ms, Rt
背景介绍

  • Endothelial nitric oxide synthase (eNOS), also known as nitric oxide synthase 3 (NOS3), is a critical enzyme primarily expressed in vascular endothelial cells, as well as in other tissues such as the myocardium, airway epithelium, and renal tubular cells. It catalyzes the conversion of L-arginine to L-citrulline, producing nitric oxide (NO) as a byproduct, which is a key signaling molecule for regulating vascular tone, promoting vasodilation, and maintaining cardiovascular homeostasis. eNOS activity is regulated by various stimuli, including shear stress, calcium influx, and phosphorylation at specific residues such as serine 1177. Dysfunction of eNOS, such as uncoupling due to oxidative stress or deficiency of cofactors like tetrahydrobiopterin (BH4), can lead to reduced NO production and contribute to endothelial dysfunction, a hallmark of cardiovascular diseases.

  • 免疫印迹

    • WB result of eNOS Recombinant Rabbit mAb
      Primary antibody: eNOS Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: HeLa whole cell lysate 20 µg
      Lane 2: HUVEC whole cell lysate 20 µg
      Negative control: HeLa whole cell lysate
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 133 kDa
      Observed MW: 133 kDa

    • WB result of eNOS Recombinant Rabbit mAb
      Primary antibody: eNOS Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: mouse spleen lysate 20 µg
      Lane 2: mouse liver lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 133 kDa
      Observed MW: 133 kDa

    • WB result of eNOS Recombinant Rabbit mAb
      Primary antibody: eNOS Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: rat liver lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 133 kDa
      Observed MW: 133 kDa

  • 免疫组化

    • IHC shows positive staining in paraffin-embedded human spleen. Anti-eNOS antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human colon cancer. Anti-eNOS antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded mouse colon. Anti-eNOS antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows negative staining in paraffin-embedded mouse skeletal muscle (low expression). Anti-eNOS antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded rat colon. Anti-eNOS antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded rat kidney. Anti-eNOS antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded rat spleen. Anti-eNOS antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

  • 组织表达图谱

    • Expression of eNOS in tumor tissue.

    • Expression of eNOS in human tissue.

    • Expression of eNOS in mouse & rat tissue.

  • 斯达特公司的抗体，可以回收利用几次？

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