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Rabbit抗原名称
eNOS分子别名
Nitric oxide synthase 3; Constitutive NOS (cNOS); EC-NOS; NOS type III (NOSIII); Nitric oxide synthase; endothelial (Endothelial NOS; eNOS); NOS3免疫原
Synthetic Peptide细胞定位
Cytoplasm, Cell membraneAccession
P29474克隆号
S-1885-94抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt阳性样本
HUVEC, mouse liver, mouse spleen, rat liver纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu, Ms, Rt IHC-P 1:250 Hu, Ms, Rt
Endothelial nitric oxide synthase (eNOS), also known as nitric oxide synthase 3 (NOS3), is a critical enzyme primarily expressed in vascular endothelial cells, as well as in other tissues such as the myocardium, airway epithelium, and renal tubular cells. It catalyzes the conversion of L-arginine to L-citrulline, producing nitric oxide (NO) as a byproduct, which is a key signaling molecule for regulating vascular tone, promoting vasodilation, and maintaining cardiovascular homeostasis. eNOS activity is regulated by various stimuli, including shear stress, calcium influx, and phosphorylation at specific residues such as serine 1177. Dysfunction of eNOS, such as uncoupling due to oxidative stress or deficiency of cofactors like tetrahydrobiopterin (BH4), can lead to reduced NO production and contribute to endothelial dysfunction, a hallmark of cardiovascular diseases.
免疫印迹
WB result of eNOS Recombinant Rabbit mAb
Primary antibody: eNOS Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: HUVEC whole cell lysate 20 µg
Negative control: HeLa whole cell lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 133 kDa
Observed MW: 133 kDaWB result of eNOS Recombinant Rabbit mAb
Primary antibody: eNOS Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse spleen lysate 20 µg
Lane 2: mouse liver lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 133 kDa
Observed MW: 133 kDaWB result of eNOS Recombinant Rabbit mAb
Primary antibody: eNOS Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat liver lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 133 kDa
Observed MW: 133 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human spleen. Anti-eNOS antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-eNOS antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse colon. Anti-eNOS antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows negative staining in paraffin-embedded mouse skeletal muscle (low expression). Anti-eNOS antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat colon. Anti-eNOS antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat kidney. Anti-eNOS antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat spleen. Anti-eNOS antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of eNOS in tumor tissue.
Expression of eNOS in human tissue.
Expression of eNOS in mouse & rat tissue.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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