Non-phospho (Active) β-Catenin (Ser37/Thr41) Recombinant Rabbit mAb (S-1813-42)

CTNNB1

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B1403

规格

25μl100μl1ml

数量

产品介绍

产品介绍 FAQs 评论(0)

产品规格

宿主来源

Rabbit
抗原名称

Non-phospho (Active) β-Catenin (Ser37/Thr41)
分子别名

CTNNB1
免疫原

Synthetic Peptide
细胞定位

Cell membrane, Cytoplasm, Nucleus
Accession

P35222
克隆号

S-1813-42
抗体类型

Recombinant mAb
抗体同种型

IgG
翻译后修饰类型

磷酸化
反应种属 ?

Hu, Ms
阳性样本

HeLa, 293T, A431, NIH/3T3
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用
稀释度

应用稀释度推荐种属

Dot Blot 1:1000
WB 1:1000 Hu, Ms
IHC-P 1:500 Hu

应用	稀释度	推荐种属
Dot Blot	1:1000
WB	1:1000	Hu, Ms
IHC-P	1:500	Hu

背景介绍

Non-phospho (Active) β-Catenin (Ser37/Thr41) refers to a form of the β-catenin protein that is not phosphorylated at the serine 37 and threonine 41 residues. This unphosphorylated state stabilizes β-catenin, allowing it to accumulate in the cytoplasm and subsequently translocate to the nucleus, where it acts as a transcriptional co-activator in the Wnt signaling pathway. The presence of non-phospho β-Catenin (Ser37/Thr41) is often associated with active Wnt signaling, which plays a critical role in cell proliferation, differentiation, and development. Detection of this form is commonly used in research to assess Wnt pathway activity.

免疫印迹
- WB result of Non-phospho (Active) β-Catenin (Ser37/Thr41) Recombinant Rabbit mAb
  Primary antibody: Non-phospho (Active) β-Catenin (Ser37/Thr41) Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: HeLa whole cell lysate 20 µg
  Lane 2: 293T whole cell lysate 20 µg
  Lane 3: A431 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 85 kDa
  Observed MW: 70-100 kDa
- WB result of Non-phospho (Active) β-Catenin (Ser37/Thr41) Recombinant Rabbit mAb
  Primary antibody: Non-phospho (Active) β-Catenin (Ser37/Thr41) Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: NIH/3T3 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 85 kDa
  Observed MW: 100 kDa
斑点杂交
- Dot blot result of Non-phospho (Active) β-Catenin (Ser37/Thr41) Recombinant Rabbit mAb
  Lane 1: Non-phospho β-Catenin (Ser33/37/Thr41) peptide
  Lane 2: Phospho β-Catenin (Ser33) peptide
  Lane 3: Phospho β-Catenin (Ser37) peptide
  Lane 4: Phospho β-Catenin (Thr41) peptide
  Primary antibody: Non-phospho (Active) β-Catenin (Ser37/Thr41) Recombinant Rabbit mAb at 1/1000 dilution
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
免疫组化
- IHC shows positive staining in paraffin-embedded human colon. Anti- Non-phospho (Active) β-Catenin (Ser37/Thr41) antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human stomach. Anti- Non-phospho (Active) β-Catenin (Ser37/Thr41) antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human pancreatic cancer. Anti- Non-phospho (Active) β-Catenin (Ser37/Thr41) antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human endometrial cancer. Anti- Non-phospho (Active) β-Catenin (Ser37/Thr41) antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
- Expression of Non-phospho (Active) β-Catenin (Ser37/Thr41) in tumor tissue.
- Expression of Non-phospho (Active) β-Catenin (Ser37/Thr41) in human tissue.