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别名
beta-Catenin,CTNB1,CTNNB1,CTNNB,OK/SW-cl.35,PRO2286,β-Catenin,βCatenin,b-Catenin,beta Catenin,betaCatenin,Catenin-β宿主来源
Rabbit抗原名称
Non-phospho (Active) β-Catenin (Ser37/Thr41)免疫原
Synthetic Peptide细胞定位
Cell membrane, Cytoplasm, NucleusAccession
P35222克隆号
S-1813-42抗体类型
Recombinant mAb抗体同种型
IgG应用
IHC-P, WB反应种属 ?
Hu, Ms阳性样本
HeLa, 293T, A431, NIH/3T3纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
| 应用 | 稀释度 | 推荐种属 |
|---|---|---|
| Dot Blot | 1:1000 | |
| WB | 1:1000 | Hu, Ms |
| IHC-P | 1:500 | Hu |
Non-phospho (Active) β-Catenin (Ser37/Thr41) refers to a form of the β-catenin protein that is not phosphorylated at the serine 37 and threonine 41 residues. This unphosphorylated state stabilizes β-catenin, allowing it to accumulate in the cytoplasm and subsequently translocate to the nucleus, where it acts as a transcriptional co-activator in the Wnt signaling pathway. The presence of non-phospho β-Catenin (Ser37/Thr41) is often associated with active Wnt signaling, which plays a critical role in cell proliferation, differentiation, and development. Detection of this form is commonly used in research to assess Wnt pathway activity.
免疫印迹
WB result of Non-phospho (Active) β-Catenin (Ser37/Thr41) Recombinant Rabbit mAb
Primary antibody: Non-phospho (Active) β-Catenin (Ser37/Thr41) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: 293T whole cell lysate 20 µg
Lane 3: A431 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 85 kDa
Observed MW: 70-100 kDaWB result of Non-phospho (Active) β-Catenin (Ser37/Thr41) Recombinant Rabbit mAb
Primary antibody: Non-phospho (Active) β-Catenin (Ser37/Thr41) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 85 kDa
Observed MW: 100 kDa
斑点杂交
Dot blot result of Non-phospho (Active) β-Catenin (Ser37/Thr41) Recombinant Rabbit mAb
Lane 1: Non-phospho β-Catenin (Ser33/37/Thr41) peptide
Lane 2: Phospho β-Catenin (Ser33) peptide
Lane 3: Phospho β-Catenin (Ser37) peptide
Lane 4: Phospho β-Catenin (Thr41) peptide
Primary antibody: Non-phospho (Active) β-Catenin (Ser37/Thr41) Recombinant Rabbit mAb at 1/1000 dilution
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
免疫组化
IHC shows positive staining in paraffin-embedded human colon. Anti- Non-phospho (Active) β-Catenin (Ser37/Thr41) antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human stomach. Anti- Non-phospho (Active) β-Catenin (Ser37/Thr41) antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human pancreatic cancer. Anti- Non-phospho (Active) β-Catenin (Ser37/Thr41) antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human endometrial cancer. Anti- Non-phospho (Active) β-Catenin (Ser37/Thr41) antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of Non-phospho (Active) β-Catenin (Ser37/Thr41) in tumor tissue.
Expression of Non-phospho (Active) β-Catenin (Ser37/Thr41) in human tissue.
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