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Rabbit抗原名称
NDP52分子别名
Calcium-binding and coiled-coil domain-containing protein 2; Antigen nuclear dot 52 kDa protein; Nuclear domain 10 protein NDP52 (Nuclear domain 10 protein 52); Nuclear dot protein 52; CALCOCO2免疫原
Synthetic Peptide细胞定位
Cytoplasm, CytoskeletonAccession
Q13137抗体类型
Polyclonal antibody抗体同种型
IgG反应种属 ?
Hu阳性样本
HeLa, A549, THP-1纯化方式
Immunogen Affinity浓度
2 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu
NDP52, also known as CALCOCO2, is a key autophagy receptor protein involved in selective autophagy processes, playing a crucial role in the recognition and degradation of invading pathogens and damaged organelles such as mitochondria. It acts as a bridge linking target substrates to autophagosomes through interactions with ubiquitinated cargos and LC3, a component of the autophagosome membrane. NDP52 is also implicated in immune signaling, where it can regulate Toll-like receptor (TLR) pathways and has been shown to mediate antiviral responses by targeting viral components for lysosomal degradation. Additionally, NDP52 has been found to interact with various proteins, including TBK1, NAP1, and Myosin VI, highlighting its multifaceted role in cellular homeostasis and defense mechanisms.
免疫印迹
WB result of NDP52 Rabbit pAb
Primary antibody: NDP52 Rabbit pAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: A549 whole cell lysate 20 µg
Lane 3: THP-1 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 52 kDa
Observed MW: 52 kDa
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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