分享产品介绍 FAQs 评论(0)
宿主来源
Rabbit抗原名称
Beta IV Tubulin分子别名
Tubulin beta-4A chain; Tubulin 5 beta; Tubulin beta-4 chain; TUBB4; TUBB5; TUBB4A免疫原
Synthetic Peptide细胞定位
Cytoskeleton, CytoplasmAccession
P04350克隆号
S-1572-30抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt阳性样本
HeLa, K562, Jurkat, U-2 OS, mouse brain, mouse spleen, mouse kidney, C6, rat brain纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
ICFCM
IHC-P ?
ICC ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000-1:20000 Hu, Ms, Rt IHC-P 1:2000 Hu, Ms, Rt ICC 1:500 Ms ICFCM 1:500 Ms
Beta IV Tubulin is a member of the beta tubulin family, encoded by the TUBB4A gene, and is primarily involved in the formation of microtubules, which are essential components of the cytoskeleton. It plays a critical role in cellular functions such as maintaining cell shape, facilitating intracellular transport, and enabling cell division. Beta IV Tubulin is selectively expressed in oligodendrocytes in the central nervous system, where it helps transport myelin proteins, lipids, and RNA during myelination. Additionally, it is required for the proper structure and function of cilia in various tissues, including the trachea, ependyma, and testis. Mutations in the TUBB4A gene can lead to neurological disorders such as hypomyelinating leukodystrophy and dystonia.
免疫印迹
WB result of Beta IV Tubulin Recombinant Rabbit mAb
Primary antibody: Beta IV Tubulin Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: K562 whole cell lysate 20 µg
Lane 3: Jurkat whole cell lysate 20 µg
Lane 4: U-2 OS whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 50 kDa
Observed MW: 55 kDaWB result of Beta IV Tubulin Recombinant Rabbit mAb
Primary antibody: Beta IV Tubulin Recombinant Rabbit mAb at 1/20000 dilution
Lane 1: mouse brain lysate 20 µg
Lane 2: mouse spleen lysate 20 µg
Lane 3: mouse kidney lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 50 kDa
Observed MW: 50 kDaWB result of Beta IV Tubulin Recombinant Rabbit mAb
Primary antibody: Beta IV Tubulin Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: C6 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 50 kDa
Observed MW: 50 kDaWB result of Beta IV Tubulin Recombinant Rabbit mAb
Primary antibody: Beta IV Tubulin Recombinant Rabbit mAb at 1/20000 dilution
Lane 1: rat brain lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 50 kDa
Observed MW: 50 kDa
流式分析
Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized Neuro-2a (Mouse neuroblastoma neuroblast) labelling Beta IV antibody at 1/500 dilution (0.1 μg) / (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
免疫组化
IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-Beta IV Tubulin antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human testis. Anti-Beta IV Tubulin antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast cancer. Anti-Beta IV Tubulin antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-Beta IV Tubulin antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti-Beta IV Tubulin antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
ICC shows positive staining in Neuro-2a cells. Anti- Beta IV Tubulin antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
组织表达图谱
Expression of Beta IV Tubulin in tumor tissue.
Expression of Beta IV Tubulin in human tissue.
Expression of Beta IV Tubulin in mouse & rat tissue.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
评论(0)