ALDH1A1 Rabbit Polyclonal Antibody
Aldehyde dehydrogenase 1A1,3-deoxyglucosone dehydrogenase,ALDH-E1,ALHDII,Aldehyde dehydrogenase family 1 member A1,Aldehyde dehydrogenase,cytosolic,Retinal dehydrogenase 1 (RALDH 1,RalDH1),ALDC,ALDH1
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宿主来源
Rabbit抗原名称
ALDH1A1分子别名
Aldehyde dehydrogenase 1A1; 3-deoxyglucosone dehydrogenase; ALDH-E1; ALHDII; Aldehyde dehydrogenase family 1 member A1; Aldehyde dehydrogenase; cytosolic; Retinal dehydrogenase 1 (RALDH 1; RalDH1); ALDC; ALDH1免疫原
Synthetic Peptide细胞定位
CytoplasmAccession
P00352抗体类型
Polyclonal antibody抗体同种型
IgG反应种属 ?
Hu, Ms阳性样本
HepG2, HT-29, A549, mouse liver, mouse lung纯化方式
Immunogen Affinity浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu, Ms IHC-P 1:250 Hu, Ms
Aldehyde dehydrogenase 1A1 (ALDH1A1) is an NADP-dependent enzyme belonging to the ALDH family, playing a crucial role in various physiological and toxicological processes. It is widely recognized as a marker for tumor-initiating cells and is involved in maintaining the stemness of tumor cells, promoting tumor angiogenesis and metastasis, and contributing to resistance against anticancer drugs. ALDH1A1 also lowers intracellular pH in breast cancer cells, suppressing antitumor immunity and fostering cancer progression. Recent studies have shown that ALDH1A1 upregulates ZBTB7B, a transcription factor that promotes glycolysis in tumor cells, leading to increased lactate production and immune evasion. This highlights ALDH1A1's potential as a therapeutic target for cancer immunotherapy.
免疫印迹
WB result of ALDH1A1 Rabbit pAb
Primary antibody: ALDH1A1 Rabbit pAb at 1/1000 dilution
Lane 1: HepG2 whole cell lysate 20 µg
Lane 2: HT-29 whole cell lysate 20 µg
Lane 3: A549 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 55 kDa
Observed MW: 55 kDaWB result of ALDH1A1 Rabbit pAb
Primary antibody: ALDH1A1 Rabbit pAb at 1/1000 dilution
Lane 1: mouse liver lysate 20 µg
Lane 2: mouse lung lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 55 kDa
Observed MW: 50 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human kidney. Anti-ALDH1A1 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human testis. Anti-ALDH1A1 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-ALDH1A1 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human endometrial cancer. Anti-ALDH1A1 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse liver. Anti-ALDH1A1 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of ALDH1A1 in tumor tissue.
Expression of ALDH1A1 in human tissue.
Expression of ALDH1A1 in mouse & rat tissue.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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