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Rabbit抗原名称
TERT分子别名
Telomerase reverse transcriptase; HEST2; Telomerase catalytic subunit; Telomerase-associated protein 2 (TP2); EST2; TCS1; TRT免疫原
Synthetic Peptide细胞定位
Cytoplasm, NucleusAccession
O14746克隆号
S-1246-143抗体类型
Recombinant mAb抗体同种型
IgG应用
ICFCM, ICC, WB反应种属 ?
Hu阳性样本
HeLa, Jurkat, K562纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
| 应用 | 稀释度 | 推荐种属 |
|---|---|---|
| WB | 1:1000 | Hu |
| ICC | 1:500 | ICC |
| ICFCM | 1:500 | Hu |
TERT, or telomerase reverse transcriptase, is a crucial component of the enzyme telomerase, which is responsible for maintaining the length of telomeres, the protective caps at the ends of chromosomes. TERT is composed of multiple domains, including a telomerase conserved motif domain, a catalytic domain, and an RNA-binding domain. It functions by reverse transcribing its RNA template into DNA and adding it to the telomere DNA sequence, ensuring the integrity of chromosomes during cell division. TERT expression is tightly regulated in normal cells but is often upregulated in cancer cells, contributing to their uncontrolled proliferation. Besides its role in telomere maintenance, TERT is also involved in DNA repair, cell survival, and proliferation, and it has been implicated in various diseases, including cancer and pulmonary fibrosis.
免疫印迹
WB result of TERT Recombinant Rabbit mAb
Primary antibody: TERT gamma Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: Jurkat whole cell lysate 20 µg
Lane 3: K562 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 127 kDa
Observed MW: 127 kDa
流式分析
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) labelling TERT Antibody at 1/500 dilution (0.1 μg) / (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
免疫细胞化学
ICC shows positive staining in Jurkat cells. Anti-TERT antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
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