TERT Recombinant Rabbit mAb (S-1246-143)

Telomerase reverse transcriptase,HEST2,Telomerase catalytic subunit,Telomerase-associated protein 2 (TP2),EST2,TCS1,TRT

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B1395

规格

25μl100μl1ml

数量

产品介绍

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产品规格

宿主来源

Rabbit
抗原名称

TERT
分子别名

Telomerase reverse transcriptase; HEST2; Telomerase catalytic subunit; Telomerase-associated protein 2 (TP2); EST2; TCS1; TRT
免疫原

Synthetic Peptide
细胞定位

Cytoplasm, Nucleus
Accession

O14746
克隆号

S-1246-143
抗体类型

Recombinant mAb
抗体同种型

IgG
反应种属 ?

Hu
阳性样本

HeLa, Jurkat, K562
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

ICFCM

ICC ?

WB
稀释度

应用稀释度推荐种属

WB 1:1000 Hu
ICC 1:500 ICC
ICFCM 1:500 Hu

背景介绍

TERT, or telomerase reverse transcriptase, is a crucial component of the enzyme telomerase, which is responsible for maintaining the length of telomeres, the protective caps at the ends of chromosomes. TERT is composed of multiple domains, including a telomerase conserved motif domain, a catalytic domain, and an RNA-binding domain. It functions by reverse transcribing its RNA template into DNA and adding it to the telomere DNA sequence, ensuring the integrity of chromosomes during cell division. TERT expression is tightly regulated in normal cells but is often upregulated in cancer cells, contributing to their uncontrolled proliferation. Besides its role in telomere maintenance, TERT is also involved in DNA repair, cell survival, and proliferation, and it has been implicated in various diseases, including cancer and pulmonary fibrosis.

免疫印迹
- WB result of TERT Recombinant Rabbit mAb
  Primary antibody: TERT gamma Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: HeLa whole cell lysate 20 µg
  Lane 2: Jurkat whole cell lysate 20 µg
  Lane 3: K562 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 127 kDa
  Observed MW: 127 kDa
流式分析
- Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) labelling TERT Antibody at 1/500 dilution (0.1 μg) / (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
免疫细胞化学
- ICC shows positive staining in Jurkat cells. Anti-TERT antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).