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宿主来源
Rabbit抗原名称
Mitofusin 2分子别名
Mitofusin-2; Transmembrane GTPase MFN2; CPRP1; KIAA0214; MFN2免疫原
Recombinant Protein细胞定位
MitochondrionAccession
O95140克隆号
S-1783-49抗体类型
Recombinant mAb抗体同种型
IgG应用
IHC-P, ICC, WB反应种属 ?
Hu, Ms, Rt阳性样本
HeLa, HEK-293, HepG2, Jurkat, Raji, SK-OV-3, NIH/3T3, mouse brain , mouse kidney, rat brain纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
| 应用 | 稀释度 | 推荐种属 |
|---|---|---|
| WB | 1:1000 | Hu, Ms, Rt |
| IHC-P | 1:200 | Hu, Ms |
| ICC | 1:500 | Hu |
Mitofusin 2 (MFN2) is a dynamin-like GTPase protein anchored to the outer mitochondrial membrane, playing a crucial role in mitochondrial fusion, fission, and tethering to the endoplasmic reticulum (ER). It helps maintain mitochondrial morphology and function, and regulates intracellular calcium homeostasis and lipid metabolism. MFN2 is also involved in mitophagy by interacting with key proteins like PINK1 and Parkin, which mediate the selective elimination of damaged mitochondria. Mutations in the MFN2 gene are associated with Charcot–Marie–Tooth disease type 2A, a neurological disorder characterized by axonal degeneration and muscle atrophy. Additionally, MFN2 has been implicated in various cellular processes, including the regulation of the NLRP3 inflammasome and the maintenance of cell homeostasis. Its dual localization and diverse functions make it a critical regulator in both mitochondrial dynamics and interorganellar communication.
免疫印迹
WB result of Mitofusin 2 Recombinant Rabbit mAb
Primary antibody: Mitofusin 2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: HEK-293 whole cell lysate 20 µg
Lane 3: HepG2 whole cell lysate 20 µg
Lane 4: Jurkat whole cell lysate 20 µg
Lane 5: Raji whole cell lysate 20 µg
Lane 6: SK-OV-3 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 86 kDa
Observed MW: 80 kDaWB result of Mitofusin 2 Recombinant Rabbit mAb
Primary antibody: Mitofusin 2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: mouse brain lysate 20 µg
Lane 3: mouse kidney lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 86 kDa
Observed MW: 80 kDaWB result of Mitofusin 2 Recombinant Rabbit mAb
Primary antibody: Mitofusin 2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat brain lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 86 kDa
Observed MW: 80 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human colon. Anti-Mitofusin 2 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cardiac muscle. Anti-Mitofusin 2 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human skeletal muscle. Anti-Mitofusin 2 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-Mitofusin 2 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse colon. Anti-Mitofusin 2 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse cardiac muscle. Anti-Mitofusin 2 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
ICC shows positive staining in HepG2 cells. Anti-Mitofusin 2 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
组织表达图谱
Expression of Mitofusin 2 in tumor tissue.
Expression of Mitofusin 2 in human tissue.
Expression of Mitofusin 2 in mouse & rat tissue.
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