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IKK gamma Recombinant Rabbit mAb (S-1359-118) KO验证

NF-kappa-B essential modulator,NEMO,FIP-3,IkB kinase-associated protein 1 (IKKAP1),Inhibitor of nuclear factor kappa-B kinase subunit gamma (I-kappa-B kinase subunit gamma,IKK-gamma,IKKG,IkB kinase subunit gamma),NF-kappa-B essential modifier,FIP3,IKBKG

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B1393

规格

25μl100μl1ml

数量

产品介绍

产品介绍 FAQs 评论(0)

产品规格

宿主来源

Rabbit
抗原名称

IKK gamma
分子别名

NF-kappa-B essential modulator; NEMO; FIP-3; IkB kinase-associated protein 1 (IKKAP1); Inhibitor of nuclear factor kappa-B kinase subunit gamma (I-kappa-B kinase subunit gamma; IKK-gamma; IKKG; IkB kinase subunit gamma); NF-kappa-B essential modifier; FIP3; NEMO; IKBKG
免疫原

Synthetic Peptide
细胞定位

Cytoplasm, Nucleus
Accession

Q9Y6K9
克隆号

S-1359-118
抗体类型

Recombinant mAb
抗体同种型

IgG
反应种属 ?

Hu, Ms, Rt
阳性样本

HeLa, PC-3, Jurkat, K562, NIH/3T3, mouse brain, C6, rat brain
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

ICFCM

IHC-P ?

ICC ?

WB
稀释度

应用稀释度推荐种属

WB 1:1000 Hu, Ms, Rt
IHC-P 1:200 Hu, Ms, Rt
ICC 1:500 Ms
ICFCM 1:50 Ms

应用	稀释度	推荐种属
WB	1:1000	Hu, Ms, Rt
IHC-P	1:200	Hu, Ms, Rt
ICC	1:500	Ms
ICFCM	1:50	Ms

背景介绍

IKK gamma, also known as NEMO (NF-κB essential modulator), is a crucial regulatory subunit of the IκB kinase (IKK) complex. The IKK complex, which also includes the catalytic subunits IKKα and IKKβ, plays a central role in activating the NF-κB signaling pathway. IKK gamma itself does not possess kinase activity, but it is essential for the assembly and activation of the IKK complex. When cells are stimulated by various factors such as cytokines, bacterial products, or viral infections, the IKK complex is activated, leading to the phosphorylation and degradation of IκB proteins. This process releases NF-κB dimers, allowing them to translocate to the nucleus and regulate the expression of numerous genes involved in inflammation, immune responses, and cell survival. Mutations in the gene encoding IKK gamma can lead to severe immunodeficiencies and developmental disorders, highlighting its critical role in maintaining immune function and cellular homeostasis.

免疫印迹
- WB result of IKK gamma Recombinant Rabbit mAb
  Primary antibody: IKK gamma Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: Wild-type HeLa whole cell lysate 20 µg
  Lane 2: IKK gamma knockout HeLa whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted band size: 48 kDa
  Observed band size: 40, 48 kDa
- WB result of IKK gamma Recombinant Rabbit mAb
  Primary antibody: IKK gamma Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: HeLa whole cell lysate 20 µg
  Lane 2: PC-3 whole cell lysate 20 µg
  Lane 3: Jurkat whole cell lysate 20 µg
  Lane 4: K562 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 48 kDa
  Observed MW: 40, 48 kDa
- WB result of IKK gamma Recombinant Rabbit mAb
  Primary antibody: IKK gamma Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: NIH/3T3 whole cell lysate 20 µg
  Lane 2: mouse brain lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 48 kDa
  Observed MW: 40, 48 kDa
- WB result of IKK gamma Recombinant Rabbit mAb
  Primary antibody: IKK gamma Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: C6 whole cell lysate 20 µg
  Lane 2: rat brain lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 48 kDa
  Observed MW: 40, 48 kDa
流式分析
- Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized NIH/3T3 (Mouse embryonic fibroblast) labelled with anti-IKK gamma antibody at 1/50 dilution (1 μg) / (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
免疫组化
- IHC shows positive staining in paraffin-embedded human testis. Anti-IKK gamma antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human breast cancer. Anti-IKK gamma antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded mouse spleen. Anti-IKK gamma antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded rat colon. Anti-IKK gamma antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
- ICC shows positive staining in NIH/3T3 cells. Anti-IKK gamma antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
组织表达图谱
- Expression of IKK gamma in tumor tissue.
- Expression of IKK gamma in human tissue.
- Expression of IKK gamma in mouse & rat tissue.