IKK gamma Recombinant Rabbit mAb (S-1359-118)
NF-kappa-B essential modulator,NEMO,FIP-3,IkB kinase-associated protein 1 (IKKAP1),Inhibitor of nuclear factor kappa-B kinase subunit gamma (I-kappa-B kinase subunit gamma,IKK-gamma,IKKG,IkB kinase subunit gamma),NF-kappa-B essential modifier,FIP3,IKBKG
货号： S0B1393
- 价格： ￥600
- 规格：
- 数量：
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宿主来源
Rabbit抗原名称
IKK gamma分子别名
NF-kappa-B essential modulator; NEMO; FIP-3; IkB kinase-associated protein 1 (IKKAP1); Inhibitor of nuclear factor kappa-B kinase subunit gamma (I-kappa-B kinase subunit gamma; IKK-gamma; IKKG; IkB kinase subunit gamma); NF-kappa-B essential modifier; FIP3; NEMO; IKBKG免疫原
Synthetic Peptide细胞定位
Nucleus, CytoplasmAccession
Q9Y6K9克隆号
S-1359-118抗体类型
Recombinant mAb抗体同种型
IgG应用
IHC-P, WB反应种属 ?
Hu, Ms, Rt阳性样本
HeLa, PC-3, Jurkat, K562, NIH/3T3, mouse brain, C6, rat brain纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
| 应用 | 稀释度 | 推荐种属 |
|---|---|---|
| WB | 1:1000 | Hu, Ms, Rt |
| IHC-P | 1:200 | Hu, Ms, Rt |
IKK gamma, also known as NEMO (NF-κB essential modulator), is a crucial regulatory subunit of the IκB kinase (IKK) complex. The IKK complex, which also includes the catalytic subunits IKKα and IKKβ, plays a central role in activating the NF-κB signaling pathway. IKK gamma itself does not possess kinase activity, but it is essential for the assembly and activation of the IKK complex. When cells are stimulated by various factors such as cytokines, bacterial products, or viral infections, the IKK complex is activated, leading to the phosphorylation and degradation of IκB proteins. This process releases NF-κB dimers, allowing them to translocate to the nucleus and regulate the expression of numerous genes involved in inflammation, immune responses, and cell survival. Mutations in the gene encoding IKK gamma can lead to severe immunodeficiencies and developmental disorders, highlighting its critical role in maintaining immune function and cellular homeostasis.
免疫印迹
WB result of IKK gamma Recombinant Rabbit mAb
Primary antibody: IKK gamma Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: PC-3 whole cell lysate 20 µg
Lane 3: Jurkat whole cell lysate 20 µg
Lane 4: K562 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 48 kDa
Observed MW: 55 kDaWB result of IKK gamma Recombinant Rabbit mAb
Primary antibody: IKK gamma Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: mouse brain lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 48 kDa
Observed MW: 55 kDaWB result of IKK gamma Recombinant Rabbit mAb
Primary antibody: IKK gamma Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: C6 whole cell lysate 20 µg
Lane 2: rat brain lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 48 kDa
Observed MW: 55 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human testis. Anti-IKK gamma antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast cancer. Anti-IKK gamma antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse spleen. Anti-IKK gamma antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat colon. Anti-IKK gamma antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of IKK gamma in tumor tissue.
Expression of IKK gamma in human tissue.
Expression of IKK gamma in mouse & rat tissue.
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