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Rabbit抗原名称
Huntingtin分子别名
Huntington disease protein (HD protein); HD; IT15; HTT免疫原
Synthetic Peptide细胞定位
Nucleus, CytoplasmAccession
P42858克隆号
S-1481-147抗体类型
Recombinant mAb抗体同种型
IgG应用
ICC, WB反应种属 ?
Hu, Ms, Rt阳性样本
HeLa, SH-SY5Y, Neuro-2a, mouse brain, PC-12, rat brain纯化方式
Protein A浓度
2 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
| 应用 | 稀释度 | 推荐种属 |
|---|---|---|
| WB | 1:1000 | Hu, Ms, Rt |
| ICC | 1:2000 | Hu |
Huntingtin (HTT) is a large soluble protein composed of approximately 3144 amino acids, with a molecular weight of around 348 kDa. It is highly expressed in the central nervous system, particularly in neurons of the cerebral cortex and hippocampus. Huntingtin plays crucial roles in various cellular processes, including clathrin-mediated endocytosis, axonal transport, and synaptic signal transduction. It also helps protect neurons from apoptosis.
免疫印迹
WB result of Huntingtin Recombinant Rabbit mAb
Primary antibody: Huntingtin gamma Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: SH-SY5Y whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 348 kDa
Observed MW: 348 kDa
This blot was developed with high sensitivity substrateWB result of Huntingtin Recombinant Rabbit mAb
Primary antibody: Huntingtin gamma Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: Neuro-2a whole cell lysate 20 µg
Lane 2: mouse brain lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 348 kDa
Observed MW: 348 kDa
This blot was developed with high sensitivity substrateWB result of Huntingtin Recombinant Rabbit mAb
Primary antibody: Huntingtin gamma Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: PC-12 whole cell lysate 20 µg
Lane 2: rat brain lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 348 kDa
Observed MW: 348 kDa
This blot was developed with high sensitivity substrate
免疫细胞化学
ICC shows positive staining in SH-SY5Y cells. Anti- Huntingtin antibody was used at 1/2000 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
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