CSF-1R Rabbit Polyclonal Antibody
CD116,CSF2RA,alphaGMR,CSF2R,CSF2RY,CSF-1 receptor,CSF-1-R,CSF-1R,M-CSF-R,Proto-oncogene c-Fms,GM-CSF-R-alpha,GMCSFR-alpha,GMR-alpha,Macrophage colony-stimulating factor 1 receptor,CSF-1 receptor (CSF-1-R,M-CSF-R),CD115,Csfmr,Fms,Csf1r
货号： S0B1391
- 价格： ￥600
- 规格：
- 数量：
大包装询价 产品介绍 评论(0)
宿主来源
Rabbit抗原名称
CSF-1R分子别名
Macrophage colony-stimulating factor 1 receptor; CSF-1 receptor (CSF-1-R; CSF-1R; M-CSF-R); Proto-oncogene c-Fms; CD115; Csfmr; Fms; Csf1r免疫原
Recombinant Protein细胞定位
Cell membraneAccession
P09581抗体类型
Polyclonal antibody抗体同种型
IgG应用
IHC-P, WB反应种属 ?
Ms阳性样本
RAW264.7, mouse spleen纯化方式
Immunogen Affinity浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
| 应用 | 稀释度 | 推荐种属 |
|---|---|---|
| WB | 1:1000 | Ms |
| IHC-P | 1:200 | Ms |
Colony stimulating factor 1 receptor (CSF1R), also known as macrophage colony-stimulating factor receptor (M-CSFR), and CD115 (Cluster of Differentiation 115), is a cell-surface protein encoded by the human CSF1R gene (known also as c-FMS). CSF1R is a receptor that can be activated by two ligands: colony stimulating factor 1 (CSF-1) and interleukin-34 (IL-34). CSF1R is highly expressed in myeloid cells, and CSF1R signaling is necessary for the survival, proliferation, and differentiation of many myeloid cell types in vivo and in vitro. CSF1R signaling is involved in many diseases and is targeted in therapies for cancer, neurodegeneration, and inflammatory bone diseases.
免疫印迹
WB result of CSF-1R Rabbit pAb
Primary antibody: CSF1R Rabbit pAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: RAW264.7 whole cell lysate 20 µg
Lane 3: mouse spleen lysate 20 µg
Negative control: NIH/3T3 whole cell lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 109 kDa
Observed MW: 130, 190 kDa
免疫组化
IHC shows positive staining in paraffin-embedded mouse spleen. Anti- CSF-1R antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse liver. Anti- CSF-1R antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of CSF-1R in mouse & rat tissue.
评论(0)