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CSF-1R Rabbit Polyclonal Antibody

Macrophage colony-stimulating factor 1 receptor,CSF-1 receptor (CSF-1-R,CSF-1R,M-CSF-R),Proto-oncogene c-Fms,CD115,Csfmr,Fms,Csf1r

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B1391

规格

25μl100μl1ml

数量

产品介绍

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产品规格

宿主来源

Rabbit
抗原名称

CSF-1R
分子别名

Macrophage colony-stimulating factor 1 receptor; CSF-1 receptor (CSF-1-R; CSF-1R; M-CSF-R); Proto-oncogene c-Fms; CD115; Csfmr; Fms; Csf1r
免疫原

Recombinant Protein
细胞定位

Cell membrane
Accession

P09581
抗体类型

Polyclonal antibody
抗体同种型

IgG
反应种属 ?

Ms
阳性样本

RAW264.7, mouse spleen
纯化方式

Immunogen Affinity
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

IHC-P ?

WB
稀释度

应用稀释度推荐种属

WB 1:1000 Ms
IHC-P 1:200 Ms

应用	稀释度	推荐种属
WB	1:1000	Ms
IHC-P	1:200	Ms

背景介绍

Colony stimulating factor 1 receptor (CSF1R), also known as macrophage colony-stimulating factor receptor (M-CSFR), and CD115 (Cluster of Differentiation 115), is a cell-surface protein encoded by the human CSF1R gene (known also as c-FMS). CSF1R is a receptor that can be activated by two ligands: colony stimulating factor 1 (CSF-1) and interleukin-34 (IL-34). CSF1R is highly expressed in myeloid cells, and CSF1R signaling is necessary for the survival, proliferation, and differentiation of many myeloid cell types in vivo and in vitro. CSF1R signaling is involved in many diseases and is targeted in therapies for cancer, neurodegeneration, and inflammatory bone diseases.

免疫印迹
- WB result of CSF-1R Rabbit pAb
  Primary antibody: CSF1R Rabbit pAb at 1/1000 dilution
  Lane 1: NIH/3T3 whole cell lysate 20 µg
  Lane 2: RAW264.7 whole cell lysate 20 µg
  Lane 3: mouse spleen lysate 20 µg
  Negative control: NIH/3T3 whole cell lysate
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 109 kDa
  Observed MW: 130, 190 kDa
免疫组化
- IHC shows positive staining in paraffin-embedded mouse spleen. Anti- CSF-1R antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded mouse liver. Anti- CSF-1R antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
- Expression of CSF-1R in mouse & rat tissue.