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宿主来源
Rabbit抗原名称
ISL2分子别名
Insulin gene enhancer protein ISL-2; Islet-2免疫原
Synthetic Peptide细胞定位
NucleusAccession
Q96A47抗体类型
Polyclonal antibody抗体同种型
IgG反应种属 ?
Hu阳性样本
HUVEC, HeLa, 293T纯化方式
Immunogen Affinity浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu IHC-P 1:200 Hu, Ms, Rt
Islet-2 (ISL2) is a LIM/homeodomain-type transcription factor that plays a crucial role in various developmental processes, particularly in the nervous system and pancreas. It is expressed in embryonic zebrafish and is involved in the differentiation of neurons and the regulation of gene expression. ISL2 is also implicated in the regulation of metabolic pathways and immune responses, and its expression is associated with diseases such as Fraser Syndrome 1 and AMME Complex. In pancreatic islets, ISL2 has been shown to be involved in the regulation of insulin secretion and β-cell function. Additionally, ISL2 has been identified as a potential tumor suppressor in pancreatic cancer, where its epigenetic silencing can reprogram the metabolism of cancer cells, leading to increased oxidative phosphorylation and sensitivity to mitochondrial complex I inhibitors.
免疫印迹
WB result of ISL2 Rabbit pAb
Primary antibody: ISL2 Rabbit pAb at 1/1000 dilution
Lane 1: HUVEC whole cell lysate 20 µg
Lane 2: HeLa whole cell lysate 20 µg
Lane 3: 293T whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 40 kDa
Observed MW: 50 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human colon. Anti-ISL2 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human liver. Anti-ISL2 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human tonsil. Anti-ISL2 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-ISL2 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse spleen. Anti-ISL2 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat spleen. Anti-ISL2 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of ISL2 in tumor tissue.
Expression of ISL2 in human tissue.
Expression of ISL2 in mouse & rat tissue.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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