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Rabbit抗原名称
FUNDC1分子别名
FUN14 domain-containing protein 1免疫原
Synthetic Peptide细胞定位
Mitochondrion outer membraneAccession
Q8IVP5抗体类型
Polyclonal antibody抗体同种型
IgG反应种属 ?
Hu, Ms, Rt阳性样本
HeLa, T-47D, MCF7, RAW264.7, mouse brain, C6, rat brain纯化方式
Immunogen Affinity浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu, Ms, Rt IHC-P 1:200-1:500 Hu, Ms, Rt
The FUNDC1 protein, also known as FUN14 domain containing 1, is a mitochondrial protein that plays a critical role in mitochondrial dynamics and cellular respiration. It is involved in various metabolic pathways, including fatty acid metabolism, glycolysis, and oxidative phosphorylation. FUNDC1 is highly expressed in tissues such as the brain and blood, and it is enriched in immune cells like monocytes and neutrophils. The protein is also implicated in immune responses and has been associated with processes such as antigen presentation and the regulation of immune cell functions. Additionally, FUNDC1 has been linked to the detoxification of reactive oxygen species (ROS) and is involved in the metabolism of several vitamins and amino acids.
免疫印迹
WB result of FUNDC1 Rabbit pAb
Primary antibody: FUNDC1 Rabbit pAb at 1/1000 dilution
Lane 1: THP-1 whole cell lysate 20 µg
Lane 2: HeLa whole cell lysate 20 µg
Lane 3: T-47D whole cell lysate 20 µg
Lane 4: MCF7 whole cell lysate 20 µg
Negative control: THP-1 whole cell lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 17 kDa
Observed MW: 17 kDaWB result of FUNDC1 Rabbit pAb
Primary antibody: FUNDC1 Rabbit pAb at 1/1000 dilution
Lane 1: RAW264.7 whole cell lysate 20 µg
Lane 2: mouse brain lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 17 kDa
Observed MW: 17 kDaWB result of FUNDC1 Rabbit pAb
Primary antibody: FUNDC1 Rabbit pAb at 1/1000 dilution
Lane 1: C6 whole cell lysate 20 µg
Lane 2: rat brain lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 17 kDa
Observed MW: 17 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-FUNDC1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cardiac muscle. Anti-FUNDC1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human pancreatic cancer. Anti-FUNDC1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-FUNDC1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat cardiac muscle. Anti-FUNDC1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of FUNDC1 in tumor tissue.
Expression of FUNDC1 in human tissue.
Expression of FUNDC1 in mouse & rat tissue.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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