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Rabbit抗原名称
MYPT1分子别名
Protein phosphatase 1 regulatory subunit 12A; Myosin phosphatase-targeting subunit 1 (Myosin phosphatase target subunit 1); Protein phosphatase myosin-binding subunit; PPP1R12A; MBS免疫原
Recombinant ProteinAccession
O14974克隆号
S-1298-106抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms阳性样本
HeLa, Caco-2, NIH/3T3纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu, Ms
MYPT1, or Myosin Phosphatase Targeting Subunit 1, is a regulatory subunit that binds to the catalytic subunit of protein phosphatase 1 (PP1) to form the myosin phosphatase (MP) complex. This complex is crucial for the dephosphorylation of myosin light chains, which regulates muscle contraction and relaxation. MYPT1 acts as a tumor suppressor in various cancers. It inhibits tumor progression by regulating gene expression and modulating the activity of oncogenic pathways. For instance, in prostate cancer, reduced MYPT1 expression is associated with increased tumor angiogenesis and poor patient prognosis. Similarly, in non-small cell lung cancer, MYPT1 regulated by miR-19b-3p inhibits tumor progression.
免疫印迹
WB result of MYPT1 Recombinant Rabbit mAb
Primary antibody: MYPT1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: Caco-2 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 115 kDa
Observed MW: 140 kDaWB result of MYPT1 Recombinant Rabbit mAb
Primary antibody: MYPT1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 115 kDa
Observed MW: 140 kDa
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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