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宿主来源
Rabbit抗原名称
MAOA分子别名
Amine oxidase [flavin-containing] A; Monoamine oxidase type A (MAO-A)免疫原
Synthetic Peptide细胞定位
Mitochondrion outer membraneAccession
P21397抗体类型
Polyclonal antibody抗体同种型
IgG应用
ICFCM, IHC-P, ICC, WB反应种属 ?
Hu, Ms阳性样本
HT-29, HepG2, LNCaP, mouse brain纯化方式
Immunogen Affinity浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
| 应用 | 稀释度 | 推荐种属 |
|---|---|---|
| WB | 1:1000 | Hu, Ms |
| IHC-P | 1:1000 | Hu |
| ICC | 1:500 | Hu |
| ICFCM | 1:500 | Hu |
MAOA, or monoamine oxidase A, is an enzyme encoded by the MAOA gene. It plays a crucial role in the breakdown of various neurotransmitters, including serotonin, dopamine, and norepinephrine. This enzyme is essential for maintaining the balance and stability of neurotransmitter levels in the brain. MAOA is a key enzyme involved in neurotransmitter regulation, behavioral control, and stress response. Its role in cancer and the potential use of MAOIs in cancer treatment highlight its importance in both neurological and oncological contexts.
免疫印迹
WB result of MAOA Rabbit pAb
Primary antibody: MAOA Rabbit pAb at 1/1000 dilution
Lane 1: HT-29 whole cell lysate 20 µg
Lane 2: HepG2 whole cell lysate 20 µg
Lane 3: LNCaP whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 60 kDa
Observed MW: 62 kDaWB result of MAOA Rabbit pAb
Primary antibody: MAOA Rabbit pAb at 1/1000 dilution
Lane 1: mouse brain lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 60 kDa
Observed MW: 62 kDa
流式分析
Flow cytometric analysis of LNCAP (Human prostate carcinoma epithelial cell) labelling MAOA antibody at 1/500 dilution (0.1 μg) / (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
免疫组化
IHC shows positive staining in paraffin-embedded human kidney. Anti-MAOA antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human liver. Anti-MAOA antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-MAOA antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human hepatocellular carcinoma. Anti-MAOA antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
ICC shows positive staining in LNCap cells. Anti- MAOA antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
组织表达图谱
Expression of MAOA in tumor tissue.
Expression of MAOA in human tissue.
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