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IκBβ Recombinant Rabbit mAb (S-810-203)

NF-kappa-B inhibitor beta,NF-kappa-BIB,I-kappa-B-beta (IkB-B,IkB-beta,IkappaBbeta),Thyroid receptor-interacting protein 9 (TR-interacting protein 9,TRIP-9),NFKBIB,IKBB,TRIP9

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B1375

规格

25μl100μl1ml

数量

产品介绍

产品介绍 FAQs 评论(0)

产品规格

宿主来源

Rabbit
抗原名称

IκBβ
分子别名

NF-kappa-B inhibitor beta; NF-kappa-BIB; I-kappa-B-beta (IkB-B; IkB-beta; IkappaBbeta); Thyroid receptor-interacting protein 9 (TR-interacting protein 9; TRIP-9); NFKBIB; IKBB; TRIP9
免疫原

Synthetic Peptide
细胞定位

Cytoplasm, Nucleus
Accession

Q15653
克隆号

S-810-203
抗体类型

Recombinant mAb
抗体同种型

IgG
反应种属 ?

Hu, Ms
阳性样本

Jurkat, HeLa, PANC-1, HDLM-2, MCF7, A20, EL4, mouse kidney
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

ICC ?

WB
稀释度

应用稀释度推荐种属

WB 1:1000 Hu, Ms
ICC 1:500 Hu

应用	稀释度	推荐种属
WB	1:1000	Hu, Ms
ICC	1:500	Hu

背景介绍

IκBβ is a member of the IκB family of proteins, which play a crucial role in regulating the activity of NF-κB transcription factors. IκBβ, like IκBα, can sequester NF-κB dimers in the cytoplasm, preventing their translocation to the nucleus and thus inhibiting the expression of NF-κB-dependent genes. Upon stimulation by lipopolysaccharide (LPS), IκBβ is slowly degraded and re-synthesized as a hypophosphorylated form that can enter the nucleus. This nuclear IκBβ can bind to NF-κB dimers such as p65:c-Rel, stabilizing them and promoting the expression of specific pro-inflammatory genes like TNF-α. Interestingly, IκBβ−/− mice show a dramatic reduction in TNF-α production in response to LPS and are resistant to LPS-induced septic shock and collagen-induced arthritis. This suggests that IκBβ not only acts as an inhibitor of NF-κB but also as a positive regulator of certain NF-κB-dependent genes, highlighting its dual role in the inflammatory response.

免疫印迹
- WB result of IκBβ Recombinant Rabbit mAb
  Primary antibody: IκBβ Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: Jurkat whole cell lysate 20 µg
  Lane 2: HeLa whole cell lysate 20 µg
  Lane 3: PANC-1 whole cell lysate 20 µg
  Lane 4: HDLM-2 whole cell lysate 20 µg
  Lane 5: MCF7 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 38 kDa
  Observed MW: 47 kDa
  This blot was developed with high sensitivity substrate
- WB result of IκBβ Recombinant Rabbit mAb
  Primary antibody: IκBβ Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: A20 whole cell lysate 20 µg
  Lane 2: EL4 whole cell lysate 20 µg
  Lane 3: mouse kidney lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 38 kDa
  Observed MW: 47 kDa
  This blot was developed with high sensitivity substrate
免疫细胞化学
- ICC shows positive staining in MCF7 cells. Anti- IκBβ antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).