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Rabbit抗原名称
ASGR1分子别名
Asialoglycoprotein receptor 1; ASGP-R 1; C-type lectin domain family 4 member H1; ASGPR 1; Hepatic lectin H1 (HL-1); CLEC4H1免疫原
Synthetic Peptide细胞定位
MembraneAccession
P07306抗体类型
Polyclonal antibody抗体同种型
IgG反应种属 ?
Hu阳性样本
HepG2纯化方式
Immunogen Affinity浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu IHC-P 1:500 Hu
The ASGR1 protein, also known as the asialoglycoprotein receptor 1, is a key component of the asialoglycoprotein receptor complex, which is involved in the recognition and uptake of glycoproteins with terminal galactose or N-acetylgalactosamine residues. This receptor is primarily expressed in hepatocytes and plays a crucial role in the clearance of circulating glycoproteins, contributing to the maintenance of homeostasis. The ASGR1 gene is located on chromosome 17 and encodes a 46 kDa glycoprotein that forms a complex with the ASGR2 subunit. Recent studies have also highlighted the potential role of ASGR1 in various metabolic processes and its implications in diseases such as atherosclerosis.
免疫印迹
WB result of ASGR1 Rabbit pAb
Primary antibody: ASGR1 Rabbit pAb at 1/1000 dilution
Lane 1: HepG2 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 33 kDa
Observed MW: 50 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human liver. Anti-ASGR1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human tonsil. Anti-ASGR1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human placenta. Anti-ASGR1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human hepatocellular carcinoma. Anti-ASGR1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human ovarian cancer. Anti-ASGR1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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