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Rabbit抗原名称
ASC/TMS1分子别名
Apoptosis-associated speck-like protein containing a CARD; mASC; PYD and CARD domain-containing protein; Pycard免疫原
Recombinant Protein细胞定位
Mitochondrion, Endoplasmic reticulum, Cytoplasm, NucleusAccession
Q9EPB4抗体类型
Polyclonal antibody抗体同种型
IgG反应种属 ?
Ms, Rt阳性样本
J774A.1, mouse spleen, rat spleen, rat colon纯化方式
Immunogen Affinity浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ? ,WB稀释度
应用 稀释度 推荐种属 WB 1:1000 Ms, Rt IHC-P 1:200 Ms, Rt
The ASC protein, also known as apoptosis-associated speck-like protein containing a caspase recruitment domain (CARD), is a 22 kDa protein that serves as a central adaptor for inflammasome assembly. It is composed of two primary domains: the pyrin domain (PYD) and the caspase recruitment domain (CARD), which facilitate its role in immune signaling. ASC is crucial for the formation of the inflammasome complex, which is activated in response to pathogen-associated molecular patterns (PAMPs) or damage-associated molecular patterns (DAMPs). This activation leads to the recruitment and activation of pro-caspase-1, resulting in the maturation and release of pro-inflammatory cytokines such as interleukin-1β (IL-1β) and interleukin-18 (IL-18). The ASC protein is expressed in various immune and non-immune cells and plays a significant role in immune homeostasis and disease progression, including cancer, bacterial and viral infections, and inflammatory diseases.
免疫印迹
WB result of ASC/TMS1 Rabbit pAb
Primary antibody: ASC/TMS1 Rabbit pAb at 1/1000 dilution
Lane 1: RAW264.7 whole cell lysate 20 µg
Lane 2: J774A.1 whole cell lysate 20 µg
Lane 3: mouse spleen lysate 20 µg
Negative control: RAW264.7 whole cell lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 21 kDa
Observed MW: 22 kDaWB result of ASC/TMS1 Rabbit pAb
Primary antibody: ASC/TMS1 Rabbit pAb at 1/1000 dilution
Lane 1: rat spleen lysate 20 µg
Lane 2: rat colon lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 21 kDa
Observed MW: 22 kDa
免疫组化
IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-ASC/TMS1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse colon. Anti-ASC/TMS1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse skeletal muscle (low expression). Anti-ASC/TMS1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse stomach. Anti-ASC/TMS1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat colon. Anti-ASC/TMS1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat spleen. Anti-ASC/TMS1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat skeletal muscle (low expression). Anti-ASC/TMS1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse stomach. Anti-ASC/TMS1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of ASC/TMS1 in mouse & rat tissue.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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